Petrenko V A, Kipriianov S M, Boldyrev A N, Pozdniakov P I
Mol Gen Mikrobiol Virusol. 1989 Mar(3):35-9.
A new approach is proposed to obtain the directed mutations in the gene under study. The technique is based on using alkylphosphotriester analogues of oligodeoxyribonucleotides as site-specific mutagens. The deletion C in lacZ' gene of bacteriophage M13mpB was obtained by cotransfection of Escherichia coli cells with a mix of DNA and phosphotriester analogues of oligonucleotides.
提出了一种获得所研究基因定向突变的新方法。该技术基于使用寡脱氧核糖核苷酸的烷基磷酸三酯类似物作为位点特异性诱变剂。通过将大肠杆菌细胞与DNA和寡核苷酸磷酸三酯类似物的混合物共转染，获得了噬菌体M13mpB的lacZ'基因中的缺失C。
