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在培养的大鼠胎儿肝细胞对胰岛素的急性反应过程中,葡萄糖、半乳糖和果糖作为糖原前体的比较作用。

Compared roles of glucose, galactose and fructose as glycogen precursors during the acute response to insulin in cultured rat foetal hepatocytes.

作者信息

Menuelle P, M'zali H, Forest N, Plas C

机构信息

Laboratoire Interactions Cellulaires, UER Odontologie, Université Paris, France.

出版信息

Int J Biochem. 1988;20(8):777-82. doi: 10.1016/0020-711x(88)90063-8.

DOI:10.1016/0020-711x(88)90063-8
PMID:3049181
Abstract
  1. The efficiency of the contribution of hexoses to basal- and stimulated-glycogenesis, when studied in cultured 18 day-old rat foetal hepatocytes in the presence of glucose, was as follows: galactose greater than glucose greater than fructose. 2. Glucose deprivation had opposite effects on the contributions of [14C]galactose (decreased) and [14C]fructose (increased) to glycogenesis, which occurred independently of insulin and were reversed by glucose concentrations as low as 30-100 microM. 3. The stimulation of glycogenesis by insulin measured with [14C]glucose (3.2-fold) was superior to that obtained with either [14C]galactose or [14C]fructose (2.7-fold in both cases), which revealed a specific beneficial effect of insulin on glucose contribution.
摘要
  1. 在葡萄糖存在的情况下,对培养的18日龄大鼠胎儿肝细胞中己糖对基础糖原生成和刺激糖原生成的贡献效率进行研究时,结果如下:半乳糖>葡萄糖>果糖。2. 葡萄糖剥夺对[14C]半乳糖(降低)和[14C]果糖(增加)对糖原生成的贡献有相反的影响,这与胰岛素无关,并且在低至30 - 100微摩尔的葡萄糖浓度下即可逆转。3. 用[14C]葡萄糖测定的胰岛素对糖原生成的刺激作用(3.2倍)优于用[14C]半乳糖或[14C]果糖获得的刺激作用(两种情况下均为2.7倍),这揭示了胰岛素对葡萄糖贡献的特定有益作用。

相似文献

1
Compared roles of glucose, galactose and fructose as glycogen precursors during the acute response to insulin in cultured rat foetal hepatocytes.在培养的大鼠胎儿肝细胞对胰岛素的急性反应过程中,葡萄糖、半乳糖和果糖作为糖原前体的比较作用。
Int J Biochem. 1988;20(8):777-82. doi: 10.1016/0020-711x(88)90063-8.
2
Differences between glucose and insulin stimulation of glycogenesis in cultured fetal hepatocytes.培养的胎儿肝细胞中葡萄糖和胰岛素刺激糖原生成的差异。
Biochim Biophys Acta. 1987 May 18;928(3):332-40. doi: 10.1016/0167-4889(87)90193-5.
3
Pathways of glycogen synthesis from glucose during the glycogenic response to insulin in cultured foetal hepatocytes.培养的胎儿肝细胞在对胰岛素的糖原生成反应过程中,由葡萄糖合成糖原的途径。
Biochem J. 1989 Nov 1;263(3):889-95. doi: 10.1042/bj2630889.
4
Role of serine biosynthesis and its utilization in the alternative pathway from glucose to glycogen during the response to insulin in cultured foetal-rat hepatocytes.丝氨酸生物合成及其在培养的胎鼠肝细胞对胰岛素反应过程中从葡萄糖到糖原的替代途径中的利用作用。
Biochem J. 1991 Jun 15;276 ( Pt 3)(Pt 3):577-82. doi: 10.1042/bj2760577.
5
Time-dependent effects of insulin on lipid synthesis in cultured fetal rat hepatocytes: a comparison between lipogenesis and glycogenesis.胰岛素对培养的胎鼠肝细胞脂质合成的时间依赖性影响:脂肪生成与糖原生成的比较
Metabolism. 1997 Apr;46(4):345-54. doi: 10.1016/s0026-0495(97)90045-7.
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Enhancement of glycogen concentrations in primary cultures of rat hepatocytes exposed to glucose and fructose.暴露于葡萄糖和果糖的大鼠肝细胞原代培养物中糖原浓度的提高。
Biochem J. 1988 May 1;251(3):795-802. doi: 10.1042/bj2510795.
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Receptor-mediated insulin degradation and insulin-stimulated glycogenesis in cultured foetal hepatocytes.培养的胎儿肝细胞中受体介导的胰岛素降解及胰岛素刺激的糖原生成
Biochem J. 1982 Feb 15;202(2):333-41. doi: 10.1042/bj2020333.
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Metabolic and secretory response of tumoral-insulin producing cells to D-fructose and D-galactose.肿瘤性胰岛素产生细胞对D-果糖和D-半乳糖的代谢及分泌反应。
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Dietary and hormonal effects on gluconeogenesis and glycogenesis from pyrute-3-14C, fructose-U-14C and glycerol-2-14C in the rat.膳食和激素对大鼠中由3-14C丙酮酸、U-14C果糖和2-14C甘油生成糖异生和糖原生成的影响。
Endocrinology. 1970 Jun;86(6):1264-71. doi: 10.1210/endo-86-6-1264.
10
The contribution of pyruvate cycling to loss of [6-3H]glucose during conversion of glucose to glycogen in hepatocytes: effects of insulin, glucose and acinar origin of hepatocytes.在肝细胞中将葡萄糖转化为糖原的过程中,丙酮酸循环对[6-3H]葡萄糖损失的贡献:胰岛素、葡萄糖及肝细胞腺泡来源的影响。
Biochem J. 1993 Jan 1;289 ( Pt 1)(Pt 1):255-62. doi: 10.1042/bj2890255.

引用本文的文献

1
Pathways of glycogen synthesis from glucose during the glycogenic response to insulin in cultured foetal hepatocytes.培养的胎儿肝细胞在对胰岛素的糖原生成反应过程中,由葡萄糖合成糖原的途径。
Biochem J. 1989 Nov 1;263(3):889-95. doi: 10.1042/bj2630889.
2
Role of serine biosynthesis and its utilization in the alternative pathway from glucose to glycogen during the response to insulin in cultured foetal-rat hepatocytes.丝氨酸生物合成及其在培养的胎鼠肝细胞对胰岛素反应过程中从葡萄糖到糖原的替代途径中的利用作用。
Biochem J. 1991 Jun 15;276 ( Pt 3)(Pt 3):577-82. doi: 10.1042/bj2760577.
3
Variations in the antagonistic effects of insulin and glucagon on glycogen metabolism in cultured foetal hepatocytes.
胰岛素和胰高血糖素对培养的胎儿肝细胞糖原代谢的拮抗作用差异。
Biochem J. 1991 Jul 1;277 ( Pt 1)(Pt 1):111-7. doi: 10.1042/bj2770111.