Campbell M A, Chader G J
Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, Bethesda, MD 20892.
Invest Ophthalmol Vis Sci. 1988 Oct;29(10):1517-22.
In vitro, as in vivo, the attachment, growth and differentiation of many cell types are dependent upon the availability of appropriate extracellular matrix (ECM) molecules. Here we have studied the effects of ECM components, including fibronectin and laminin on cultured Y-79 retinoblastoma cells. Both in 2 hr and in 3 day studies, the highest frequencies of attachment were seen with a laminin substrate (50 micrograms/35 mm culture dish). Attachment was significantly inhibited by specific anti-laminin antibodies. In longer studies of up to 1 week, laminin or fibronectin was added directly to the culture medium. Neither molecule significantly stimulated cell growth, but laminin continued to promote the highest frequencies of attachment (20% to 30% greater than any other substrate). Laminin exposure also caused morphological changes in Y-79 cells. Many cells became flattened and extended long, branching, neurite-like processes. These changes could be inhibited by inclusion of anti-laminin antibodies. Such studies may provide information about events occurring during normal eye development as well as about tumor cell attachment and growth.
在体外，如同在体内一样，许多细胞类型的附着、生长和分化都依赖于合适的细胞外基质（ECM）分子的存在。在此，我们研究了包括纤连蛋白和层粘连蛋白在内的ECM成分对培养的Y-79视网膜母细胞瘤细胞的影响。在2小时和3天的研究中，层粘连蛋白底物（50微克/35毫米培养皿）的附着频率最高。特异性抗层粘连蛋白抗体显著抑制了附着。在长达1周的更长时间研究中，将层粘连蛋白或纤连蛋白直接添加到培养基中。这两种分子均未显著刺激细胞生长，但层粘连蛋白继续促进最高的附着频率（比任何其他底物高20%至30%）。层粘连蛋白的暴露也导致Y-79细胞发生形态变化。许多细胞变得扁平并伸出长的、分支的、类似神经突的突起。这些变化可通过加入抗层粘连蛋白抗体来抑制。此类研究可能会提供有关正常眼睛发育过程中发生的事件以及肿瘤细胞附着和生长的信息。
