Nakamura M, Koshihara Y, Fujino Y, Mochizuki M, Minoda K, Masuda K
Department of Ophthalmology, University of Tokyo School of Medicine, Japan.
Jpn J Ophthalmol. 1987;31(4):598-607.
Prostaglandins (PGs) and their derivatives have been reported to modulate or inhibit a variety of tumor cells in vitro and in vivo. In the present study, an established retinoblastoma cell line, Y-79, was investigated for 1) its capacity to synthesize PGs and 2) its susceptibility to PGs and their derivative, 64E, exogenously given. The capacity of Y-79 cells to produce PGs was estimated by thin layer chromatography using [1-14C] arachidonic acid as a substrate, and it was found that no detectable amounts of PGD2, PGE2, PGF2 alpha, thromboxane B2, or 6-keto PGF1 alpha were produced by Y-79 cells. Furthermore, the effects of exogenously given PGs (PGA1, A2, D2, E1, E2, F2 alpha, and J2) and 64E on the cell proliferation of Y-79 cells in culture were examined. PGE1, E2, and F2 alpha showed no significant effects on the cell growth of Y-79 cells at all tested doses (1-20 micrograms/ml). On the other hand, PGA1, A2, D2, and J2, and 64E remarkably inhibited the cell growth of Y-79 cells. A dose-response study indicated that 64E was the most effective among these drugs, followed by PGJ2. PGD2, A1, and A2 were less effective than PGJ2. The present data demonstrate that Y-79 cells do not produce endogenous PGs, and that these cells are highly susceptible to exogenous PGs (PGJ2, D2, A1, and A2) as well as 64E.
据报道，前列腺素（PGs）及其衍生物在体外和体内可调节或抑制多种肿瘤细胞。在本研究中，我们对已建立的视网膜母细胞瘤细胞系Y-79进行了研究，以探讨其1）合成PGs的能力，以及2）对外源性给予的PGs及其衍生物64E的敏感性。以[1-14C]花生四烯酸为底物，通过薄层色谱法评估Y-79细胞产生PGs的能力，结果发现Y-79细胞未产生可检测量的PGD2、PGE2、PGF2α、血栓素B2或6-酮-PGF1α。此外，还检测了外源性给予的PGs（PGA1、A2、D2、E1、E2、F2α和J2）和64E对培养的Y-79细胞增殖的影响。在所有测试剂量（1-20微克/毫升）下，PGE1、E2和F2α对Y-79细胞的生长均无显著影响。另一方面，PGA1、A2、D2和J2以及64E显著抑制Y-79细胞的生长。剂量反应研究表明，64E在这些药物中最有效，其次是PGJ2。PGD2、A1和A2的效果不如PGJ2。目前的数据表明，Y-79细胞不产生内源性PGs，并且这些细胞对外源性PGs（PGJ2、D2、A1和A2）以及64E高度敏感。
