Howland C J, Wilkins B M
Department of Genetics, University of Leicester, England.
J Bacteriol. 1988 Oct;170(10):4958-9. doi: 10.1128/jb.170.10.4958-4959.1988.
The origin-of-transfer region of ColIb-P9 was inserted into a lambda prophage to give a bacterial chromosome mobilizable by the parental conjugative plasmid. The polarity of mobilization of chromosomal genes indicated that ColIb-P9 transfer is unidirectional, such that the transfer genes adjacent to oriT enter the recipient cell last.
将ColIb-P9的转移起始区插入λ原噬菌体中,以使细菌染色体能够被亲代接合质粒动员。染色体基因动员的极性表明,ColIb-P9的转移是单向的,使得与oriT相邻的转移基因最后进入受体细胞。