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随机来源的DNA序列作为大肠杆菌启动子结构的探针。

DNA sequences of random origin as probes of Escherichia coli promoter architecture.

作者信息

Horwitz M S, Loeb L A

机构信息

Department of Pathology, Joseph Gottstein Memorial Cancer Research Laboratory, University of Washington, Seattle 98195.

出版信息

J Biol Chem. 1988 Oct 15;263(29):14724-31.

PMID:3049585
Abstract

In order to better understand the role of the -35 sequence motif in transcription initiation by Escherichia coli sigma 70 RNA polymerase holoenzyme, -35 promoter elements of limited nucleotide composition have been selected from random DNA sequences. Functional promoter elements have been identified that are composed of just two nucleotide species (A,T; G,C; T,C; and T,G) and one nucleotide species (poly(dT)). From this study of 81 promoter mutations, two conclusions can be made. First, given a population of random DNA sequences, there exist sequences that form stronger -35 promoter elements than the consensus sequence. These sequences lack some features of the consensus sequence and reveal unexpected homology in the ordinarily nonconserved spacer sequence. Second, the sequence requirements at the -35 site may suggest a possible role for DNA secondary structure in the recognition of promoters by RNA polymerase.

摘要

为了更好地理解-35序列基序在大肠杆菌σ70 RNA聚合酶全酶转录起始中的作用,已从随机DNA序列中选择了核苷酸组成有限的-35启动子元件。已鉴定出仅由两种核苷酸种类(A,T;G,C;T,C;和T,G)和一种核苷酸种类(聚(dT))组成的功能性启动子元件。通过对81个启动子突变的研究,可以得出两个结论。首先,在一群随机DNA序列中,存在比共有序列形成更强-35启动子元件的序列。这些序列缺乏共有序列的某些特征,并在通常非保守的间隔序列中显示出意外的同源性。其次,-35位点的序列要求可能暗示了DNA二级结构在RNA聚合酶识别启动子中的可能作用。

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DNA sequences of random origin as probes of Escherichia coli promoter architecture.随机来源的DNA序列作为大肠杆菌启动子结构的探针。
J Biol Chem. 1988 Oct 15;263(29):14724-31.
2
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