Combinatorial libraries by cassette mutagenesis.
作者信息
Borrego B, Wienecke A, Schwienhorst A
机构信息
Department of Molecular Evolution Biology, Institute for Molecular Biotechnology, Jena, Germany.
出版信息
Nucleic Acids Res. 1995 May 25;23(10):1834-5. doi: 10.1093/nar/23.10.1834.
Abstract
摘要
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本文引用的文献
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Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA.使用M13衍生载体的寡核苷酸定向诱变：在任何DNA片段中产生点突变的高效通用方法。
Nucleic Acids Res. 1982 Oct 25;10(20):6487-500. doi: 10.1093/nar/10.20.6487.
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An integrated and simplified approach to cloning into plasmids and single-stranded phages.一种整合且简化的克隆到质粒和单链噬菌体中的方法。
Methods Enzymol. 1983;101:78-89. doi: 10.1016/0076-6879(83)01006-x.
3
Many random sequences functionally replace the secretion signal sequence of yeast invertase.许多随机序列在功能上可替代酵母转化酶的分泌信号序列。
Science. 1987 Jan 16;235(4786):312-7. doi: 10.1126/science.3541205.
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The Tetrahymena ribozyme acts like an RNA restriction endonuclease.嗜热四膜虫核酶的作用类似于RNA限制性内切酶。
Nature. 1986;324(6096):429-33. doi: 10.1038/324429a0.
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Promoters selected from random DNA sequences.从随机DNA序列中挑选的启动子。
Proc Natl Acad Sci U S A. 1986 Oct;83(19):7405-9. doi: 10.1073/pnas.83.19.7405.
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Combinatorial cassette mutagenesis as a probe of the informational content of protein sequences.组合盒式诱变作为探测蛋白质序列信息内容的手段
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A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.DNA片段的体外制备及特异性诱变的通用方法：蛋白质与DNA相互作用的研究
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