Colin J, Mader P, Volant A, Gravagna P, Dupont D, Eloy R, Norquist R E, Rowsey J, McGee D A
Centre hospitalier régional, Brest.
J Fr Ophtalmol. 1988;11(2):137-41.
Epikeratoplasty is a refractive surgery procedure described by Werblin and Kaufman which is simple, effective and reversible. However its development is hampered by the need of human corneas. We evaluated this procedure in rabbits and monkeys using lenticules of human collagen. The collagen is obtained from frozen human placentas, washed and subjected to pepsin digestion; it is contained in the supernatant of centrifugation and can be isolated by a physico-chemical treatment. 15 days after surgery the lenses of 14 rabbits were completely covered with a layer of epithelial cells. 6 months after surgery, the eyes of 14 monkeys were quiet and the lenses were covered with an epithelium made up of 2 to 5 layers of cells. The lens exhibited no inflammatory infiltration and there was no inflammatory reaction around the lens. These studies are still preliminary and longer term tests are necessary. These initial results are very encouraging, prompting us to develop the use of this type of material in refractive surgery.
角膜表层镜片术是一种由韦尔布林和考夫曼描述的屈光手术，它简单、有效且可逆。然而，其发展受到人类角膜需求的阻碍。我们使用人胶原蛋白薄片在兔子和猴子身上评估了该手术。胶原蛋白从冷冻的人胎盘中获取，经过清洗并进行胃蛋白酶消化；它存在于离心后的上清液中，可通过物理化学处理分离出来。手术后15天，14只兔子的晶状体完全被一层上皮细胞覆盖。手术后6个月，14只猴子的眼睛状况稳定，晶状体被由2至5层细胞组成的上皮覆盖。晶状体未表现出炎症浸润，晶状体周围也没有炎症反应。这些研究仍处于初步阶段，需要进行更长期的测试。这些初步结果非常令人鼓舞，促使我们在屈光手术中开发使用这种类型的材料。
