Gailitis R P, Thompson K P, Ren Q S, Morris J, Waring G O
Yerkes Regional Primate Center, Atlanta, Georgia.
Refract Corneal Surg. 1990 Nov-Dec;6(6):430-6.
We used a milliwatt continuous wave CO2 laser mounted on an operating microscope to study the feasibility of welding synthetic collagen epikeratoplasty lenticules to the cornea. In vitro studies and experimentation in rabbits and monkeys showed no welding effects using direct laser radiation with powers ranging from 17 mW to 1 W. Only tissue shrinkage was observed. Experiments using various adjunctive solders produced a temporary welding effect using 30% bovine serum albumin welded with a power of 35-45 mW, 325 microns spot size, moving the beam 5 mm/sec. The lenticules remained in place until the 4th postoperative day when epithelium grew underneath the lenticule, dislodging it. Histopathologic examination demonstrated epithelial migration over the solder displacing the epikeratoplasty lenticule and anterior stromal denaturization and disruption.
我们使用安装在手术显微镜上的毫瓦级连续波二氧化碳激光来研究将合成胶原蛋白角膜表层镜片焊接到角膜上的可行性。体外研究以及在兔子和猴子身上进行的实验表明，使用功率范围为17毫瓦至1瓦的直接激光辐射未产生焊接效果，仅观察到组织收缩。使用各种辅助焊料的实验中，使用30%牛血清白蛋白作为焊料，功率为35 - 45毫瓦，光斑尺寸为325微米，光束移动速度为5毫米/秒时产生了临时焊接效果。角膜表层镜片在原位保留至术后第4天，此时上皮细胞在镜片下方生长，将其移位。组织病理学检查显示上皮细胞在焊料上迁移，取代了角膜表层镜片，同时前基质变性和破坏。
