National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
German Federal Institute for Risk Assessment, Department of Biological Safety, Berlin, Germany.
Food Microbiol. 2019 Apr;78:99-103. doi: 10.1016/j.fm.2018.10.012. Epub 2018 Oct 22.
The reference method for Trichinella detection at meat inspection is the magnetic stirrer method (MSM) utilising HCl-pepsin for pooled sample digestion. Due to availability and quality issues with pepsin, alternative digestion methods are being offered, such as the Priocheck Trichinella AAD kit (T-AAD), based on serine endopeptidase digestion. In this study the T-AAD kit was compared to the reference method. Minced pork samples were spiked with T. spiralis muscle larvae (ML) with- and without capsule or T. pseudospiralis ML, and analysed with both tests. Test results of individually spiked test samples were analysed by generalised linear modelling. The T-AAD test kit was comparable to the reference method for the qualitative detection of T. spiralis in pigs, but not quantitatively. Overall, 94% of spiked T. spiralis were recovered using MSM against 75.2% when using T-AAD (p < 0.0001). Using the MSM 80.0% of spiked T. pseudospiralis were recovered against 20% with the T-AAD (p < 0.0001). Based on our experience with the T-AAD kit, we strongly recommend validating the method on site prior to introduction into routine diagnostic laboratories, but this will not alleviate the poor test sensitivity of the T-AAD for the detection of T. pseudospiralis.
在肉类检验中，旋毛虫检测的参考方法是利用 HCl-胃蛋白酶对混合样本进行消化的磁力搅拌器方法（MSM）。由于胃蛋白酶的可用性和质量问题，正在提供替代消化方法，例如基于丝氨酸内肽酶消化的 Priocheck 旋毛虫 AAD 试剂盒（T-AAD）。在这项研究中，将 T-AAD 试剂盒与参考方法进行了比较。用 T. spiralis 肌肉幼虫（ML）和/或无囊包或 T. pseudospiralis ML 对绞碎猪肉样品进行了加标，并用两种方法进行了分析。通过广义线性模型分析了单独加标测试样品的测试结果。T-AAD 测试试剂盒与参考方法在定性检测猪旋毛虫方面具有可比性，但定量检测则不然。总体而言，使用 MSM 回收了 94%的加标 T. spiralis，而使用 T-AAD 仅回收了 75.2%（p < 0.0001）。使用 MSM 回收了 80.0%的加标 T. pseudospiralis，而使用 T-AAD 仅回收了 20%（p < 0.0001）。根据我们使用 T-AAD 试剂盒的经验，我们强烈建议在将其引入常规诊断实验室之前在现场验证该方法，但这并不能缓解 T-AAD 检测 T. pseudospiralis 的测试灵敏度差的问题。
