Yohn J, Lehman T A, Kurian P, Ribovich M, Milo G E
Department of Physiological Chemistry, Ohio State University, Columbus 43210.
J Invest Dermatol. 1988 Oct;91(4):363-8. doi: 10.1111/1523-1747.ep12475997.
Human skin xenografts were established on the subscapular area of skin of nude (nu/nu NIH-Swiss background) mice. When treated with benzo[a]pyrene diol epoxide I (BPDE I), specific carcinogen-DNA adducts were formed. Separation and identification of these adducts by the 32P-postlabeling technique indicated that the 7R- and 7S-BPDE I-dpGp adducts were the major adducts. Xenografts pretreated with either allantoin or anthralin showed an increase in the major 7R- and 7S-BPDE I adducts compared to only BPDE I treatment. Likewise, we observed an increase in the quantity of different minor adducts. The ratios between the minor and major adducts in the pretreated grafts remained consistent with the ratio in the grafts treated with BPDE I only. We conclude that these modulators induce cells in the xenograft to enter S phase of the cell cycle. Moreover, we observed that these compounds altered the quantity of the minor carcinogen-DNA adducts without altering the overall ratios between the major 7R- and 7S-BPDE I-dpGp adducts and the minor carcinogen-DNA adducts.
将人类皮肤异种移植物移植到裸鼠（nu/nu NIH-瑞士背景）肩胛下区域的皮肤上。用苯并[a]芘二醇环氧化物I（BPDE I）处理后，会形成特定的致癌物-DNA加合物。通过32P后标记技术对这些加合物进行分离和鉴定表明，7R-和7S-BPDE I-dpGp加合物是主要加合物。与仅用BPDE I处理相比，用尿囊素或蒽林预处理的异种移植物中主要的7R-和7S-BPDE I加合物有所增加。同样，我们观察到不同次要加合物的数量也有所增加。预处理移植物中次要加合物与主要加合物的比例与仅用BPDE I处理的移植物中的比例保持一致。我们得出结论，这些调节剂诱导异种移植物中的细胞进入细胞周期的S期。此外，我们观察到这些化合物改变了次要致癌物-DNA加合物的数量，而没有改变主要的7R-和7S-BPDE I-dpGp加合物与次要致癌物-DNA加合物之间的总体比例。
