Yan Qin, Liu Xinli, Wang Yanan, Li Hongxing, Li Zhigang, Zhou Lin, Qu Yinbo, Li Zhonghai, Bao Xiaoming
Shandong Provincial Key Laboratory of Microbial Engineering, Department of Bioengineering, Qi Lu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China.
State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China.
AMB Express. 2018 Nov 29;8(1):190. doi: 10.1186/s13568-018-0720-2.
Conversion of various lignocellulosic materials into bioethanol is growing in demand but greatly depends on feedstock availability. Dairy cow manure is an agricultural waste widely distributed worldwide. This study investigated the induction of cellulases by cow manure and the conversion of cow manure materials into lignocellulosic ethanol. Alkaline NaOH pretreatment improved the accessibility of cow manure lignocellulose to enzymes followed by enzymatic hydrolysis using Penicillium oxalicum cellulases. The ethanol yields from pretreated cow manure and anaerobically digested cow manure were 0.19 and 0.13 g/g-raw biomass, respectively, using recombinant Saccharomyces cerevisiae strain LF1 designed for lignocellulosic ethanol production through simultaneous saccharification and fermentation. Fed-batch supplementation with cellulolytic enzymes and substrates after initial enzymatic hydrolysis also contributed to ethanol production up to 25.65 g/L. These results demonstrate that cow manure is a potential feedstock for inducing fungal cellulase expression and converting lignocellulose into bioethanol.
将各种木质纤维素材料转化为生物乙醇的需求日益增长，但这在很大程度上取决于原料的可得性。奶牛粪便作为一种农业废弃物，在全球范围内广泛分布。本研究调查了奶牛粪便对纤维素酶的诱导作用以及奶牛粪便材料向木质纤维素乙醇的转化。碱性NaOH预处理提高了奶牛粪便木质纤维素对酶的可及性，随后使用草酸青霉纤维素酶进行酶水解。使用为通过同步糖化发酵生产木质纤维素乙醇而设计的重组酿酒酵母菌株LF1，预处理后的奶牛粪便和厌氧消化后的奶牛粪便的乙醇产量分别为0.19和0.13 g/g-原料生物质。在初始酶水解后分批补料添加纤维素分解酶和底物也有助于乙醇产量达到25.65 g/L。这些结果表明，奶牛粪便是诱导真菌纤维素酶表达以及将木质纤维素转化为生物乙醇的潜在原料。
