Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, PR China.
Centre for Biofuels, Biotechnology Division, National Institute for Interdisciplinary Science and Technology (CSIR), Trivandrum 695019, India.
Bioresour Technol. 2020 Apr;302:122902. doi: 10.1016/j.biortech.2020.122902. Epub 2020 Jan 27.
Cellulase production, lignocellulose saccharification and bioethanol fermentation were integrated to efficiently produce bioethanol. A modified gas lift bioreactor was developed for bioethanol production by the integrated process. Cellulase production was achieved using Aspergillus niger mycelia immobilized within the reactor in wire meshes, and Saccharomyces cerevisiae cells were immobilized in resin beads. During four repeated batches fermentation, cellulase activities were more than 6.28 U/mL and bioethanol production was over 45.9 g/L for 48 h. The factual bioethanol conversion efficiency was 86.8%. By the modification of the modified gas lift bioreactor, immobilization of Aspergillus niger mycelia and Saccharomyces cerevisiae cells, aerobic cellulase production, substrate saccharification and anaerobic bioethanol fermentation were successfully integrated in tandem. The integrated processes is of great significance in bioethanol production.
细胞酶生产、木质纤维素糖化和生物乙醇发酵被集成以有效地生产生物乙醇。改良的气升式生物反应器被开发用于通过集成工艺生产生物乙醇。通过在金属网中的反应器内固定化黑曲霉菌丝体来实现纤维素酶的生产，并且将酿酒酵母细胞固定在树脂珠中。在四个重复批次发酵中，纤维素酶活性超过 6.28 U/mL，48 小时内生物乙醇产量超过 45.9 g/L。实际的生物乙醇转化率为 86.8%。通过改良气升式生物反应器的改进、黑曲霉菌丝体和酿酒酵母细胞的固定化、好氧纤维素酶生产、底物糖化和厌氧生物乙醇发酵的成功串联集成，实现了生物乙醇生产的有效集成。
