Key Laboratory of Applied Chemistry, Department of Applied Chemistry, Yanshan University, Qinhuangdao 066004, People's Republic of China.
Nanotechnology. 2019 Feb 1;30(5):055501. doi: 10.1088/1361-6528/aaee3f. Epub 2018 Nov 30.
The exploration of new materials for modifying electrodes is important to advance electrochemical biosensors. Herein, we demonstrated that amorphous bimetallic boride material (Co-2Ni-B) prepared by a simple and facile aqueous reaction is an efficient matrix to immobilize acetylcholinesterase (AChE) to construct a biosensor for the determination of organophosphate pesticides. The effects of different composition and crystallinity on its electrochemical performance are investigated, and the optimization studies of the biological transducer were also discussed. Under optimal conditions, the fabricated sensor showed good analytical performance for the determination of chlorpyrifos with a low limit of detection (2.83 pM) and a wide linear range (3 pM-300 nM). The proposed biosensor also demonstrated high reproducibility, stability and accuracy. The impressive performance was due to the excellent conductivity and the unique amorphous bimetal-metalloid complex nanostructure. These results introduce a new class of promising materials as a robust platform for biosensor applications.
探索用于修饰电极的新材料对于推进电化学生物传感器至关重要。在此，我们证明了通过简单易行的水相反应制备的非晶态双金属硼化物材料（Co-2Ni-B）是一种有效的基质，可以固定乙酰胆碱酯酶（AChE）来构建用于测定有机磷农药的生物传感器。研究了不同组成和结晶度对其电化学性能的影响，并讨论了生物换能器的优化研究。在最佳条件下，所制备的传感器对毒死蜱的测定表现出良好的分析性能，检测限低（2.83 pM），线性范围宽（3 pM-300 nM）。该生物传感器还表现出高重现性、稳定性和准确性。令人印象深刻的性能归因于其优异的导电性和独特的非晶态双金属-类金属复合纳米结构。这些结果为生物传感器应用提供了一类有前途的新材料。
