Collodel Giulia, Nerucci Fabiola, Signorini Cinzia, Iacoponi Francesca, Moretti Elena
a Department of Molecular and Developmental Medicine , University of Siena , Siena , Italy.
b Division of Clinical Pathology , University Teaching Hospital of Siena , Siena , Italy.
Syst Biol Reprod Med. 2019 Apr;65(2):155-163. doi: 10.1080/19396368.2018.1548668. Epub 2018 Nov 30.
The aim of the study was to assess whether abnormal levels of seminal biochemical components could be associated with semen alterations and infertility. In this study, 92 human ejaculates from selected men were analyzed. Albumin, estradiol, ferritin, total proteins (TP), folic acid (FA), vitamin B12, alkaline phosphatase (ALP), creatine kinase (CK), gamma-glutamyl transpeptidase (GGT), lactate dehydrogenase were evaluated. Semen parameters and biochemical components of the 92 samples were correlated bySpearman's rho coefficient. Albumin showed a negative correlation with sperm progressive motility and vitality (P < 0.05), CK with sperm concentration and vitality (P < 0.05), ferritin with sperm morphology (P < 0.05). FA negatively correlated with sperm concentration (P < 0.05) and GGT with sperm motility (P < 0.05). The values of biochemical components were compared for each semen parameters (concentration, motility, morphology, vitality) in samples ≤5th percentile with those >5th percentile and in patients with/without leukocytospermia, presence/absence of germ cells, increased/normal viscosity by Mann Whitney U test. The albumin (P < 0.001) and TP (P < 0.05) levels and the GGT activity (P < 0.001) were significantly higher in patients with sperm motility ≤5th percentile. Patients with sperm vitality ≤5th percentile showed increased albumin concentration (P < 0.01) and the CK activity (P < 0.001). The presence of germ cells in semen was concomitant with high values of ferritin (P < 0.01); the ALP activity (P < 0.01) and FA level (P < 0.001) were decreased in hyperviscous semen. The FA and estradiol levels were significantly decreased in the smoker group compared to those measured in the non-smoker group. Subjects were grouped in infertile patients and men with unknown reproductive potential. Infertile patients albumin and ferritin were significantly increased (P < 0.05). This study suggests that some biochemical components may be associated with human seminal pathological conditions. Abbreviations: ALP: alkaline phosphatase; LDH: lactate dehydrogenase; GGT: γ-glutamyl transferase; CK: creatine kinase; ACP: acid phosphatase; ALB: albumin; TP: total proteins; FERR: ferritin, E: estradiol; FOL: folic acid; B12: vitamin B12; FSH: follicle stimulating hormone; LH: luteinizing hormone; T: testosterone; BMI: body mass index; WHO: World Health Organization.
本研究的目的是评估精液生化成分异常水平是否与精液改变及不育相关。在本研究中，对选定男性的92份人类精液样本进行了分析。评估了白蛋白、雌二醇、铁蛋白、总蛋白（TP）、叶酸（FA）、维生素B12、碱性磷酸酶（ALP）、肌酸激酶（CK）、γ-谷氨酰转肽酶（GGT）、乳酸脱氢酶。通过Spearman等级相关系数对92份样本的精液参数和生化成分进行相关性分析。白蛋白与精子前向运动和活力呈负相关（P<0.05），CK与精子浓度和活力呈负相关（P<0.05），铁蛋白与精子形态呈负相关（P<0.05）。FA与精子浓度呈负相关（P<0.05），GGT与精子活力呈负相关（P<0.05）。采用Mann-Whitney U检验比较了精液参数（浓度、活力、形态、活力）处于第5百分位数及以下的样本与高于第5百分位数的样本，以及有/无白细胞精子症、有无生殖细胞、精液粘度增加/正常的患者的生化成分值。精子活力处于第5百分位数及以下的患者，其白蛋白（P<0.001）和TP（P<0.05）水平以及GGT活性（P<0.001）显著更高。精子活力处于第5百分位数及以下的患者，其白蛋白浓度（P<0.01）和CK活性（P<0.001）升高。精液中生殖细胞的存在与铁蛋白高值相关（P<0.01）；精液粘度高时，ALP活性（P<0.01）和FA水平（P<0.001）降低。与非吸烟组相比，吸烟组的FA和雌二醇水平显著降低。将受试者分为不育患者和生殖潜力未知的男性。不育患者的白蛋白和铁蛋白显著升高（P<0.05）。本研究表明，某些生化成分可能与人类精液病理状况相关。缩写：ALP：碱性磷酸酶；LDH：乳酸脱氢酶；GGT：γ-谷氨酰转移酶；CK：肌酸激酶；ACP：酸性磷酸酶；ALB：白蛋白；TP：总蛋白；FERR：铁蛋白；E：雌二醇；FOL：叶酸；B12：维生素B12；FSH：卵泡刺激素；LH：黄体生成素；T：睾酮；BMI：体重指数；WHO：世界卫生组织。
