Hsi B L, Samson M, Grivaux C, Fénichel P, Hunt J S, Yeh C J
INSERM U210, Faculté de Médecine, Nice, France.
J Reprod Immunol. 1988 Jul;13(2):183-91. doi: 10.1016/0165-0378(88)90061-7.
The expression of class I major histocompatibility complex (MHC) antigens on different regions of human amnion was studied by the avidin-biotin-complex immunoperoxidase (ABC) technique using monoclonal antibodies. In contrast to previous reports, the use of higher affinity monoclonal antibodies and the sensitive immunoperoxidase method has allowed the identification of class I MHC antigens on the amniotic epithelium. The level of expression is different between cells from various parts of the amnion, with the amniotic epithelium from the edge of the placenta consistently showing the strongest reactivity. Some of the class I MHC antigens expressed by the amnion are similar to those expressed by extravillous trophoblast cells in that both show much weaker or no reactivity with the monoclonal antibody 61D2.
采用抗生物素蛋白-生物素复合物免疫过氧化物酶（ABC）技术及单克隆抗体，研究了人羊膜不同区域Ⅰ类主要组织相容性复合体（MHC）抗原的表达情况。与先前的报道不同，使用高亲和力单克隆抗体和灵敏的免疫过氧化物酶方法已能够鉴定羊膜上皮细胞上的Ⅰ类MHC抗原。羊膜不同部位细胞的表达水平有所不同，胎盘边缘的羊膜上皮始终显示出最强的反应性。羊膜表达的一些Ⅰ类MHC抗原与绒毛外滋养层细胞表达的抗原相似，因为两者与单克隆抗体61D2的反应性都弱得多或无反应性。
