麻风衍生棒状杆菌、麻风分枝杆菌以及其他棒状杆菌和分枝杆菌的基因组大小与同源性

Size and homology of the genomes of leprosy-derived corynebacteria, Mycobacterium leprae, and other corynebacteria and mycobacteria.

作者信息

Antoine I, Coene M, Cocito C

机构信息

Microbiology and Genetics Unit, ICP, University of Louvain, Medical School, Brussels, Belgium.

出版信息

J Med Microbiol. 1988 Sep;27(1):45-50. doi: 10.1099/00222615-27-1-45.

DOI:10.1099/00222615-27-1-45

PMID:3050108

Abstract

The genomes of Mycobacterium leprae and leprosy-derived corynebacteria (LDC), which have a similar base composition of guanine + cytosine 56 mol %, have been compared with those of reference bacteria of the CMN group (genera Corynebacterium, Mycobacterium, Nocardia). Genome sizes of three LDC strains were (1.2-2.5) x 10(6) base pairs. DNA from four of seven LDC strains examined had homology levels greater than 60%. Two other strains had a homology of 40% when compared with the CMN strains and one strain was distinctly different. The DNA from all seven LDC strains gave 0.3-18% hybridisation with that of M. leprae, 5-16% with reference corynebacteria, 5-12% with M. bovis, and 2-8% with Nocardia caviae. The small size of the LDC genome and its unrelatedness to those of M. leprae and organisms of the CMN group shows the uniqueness of LDC.

摘要

麻风分枝杆菌和麻风衍生棒状杆菌（LDC）的基因组，其鸟嘌呤+胞嘧啶的碱基组成相似，为56摩尔%，已与CMN组（棒状杆菌属、分枝杆菌属、诺卡氏菌属）的参考细菌的基因组进行了比较。三种LDC菌株的基因组大小为（1.2 - 2.5）×10⁶碱基对。所检测的七株LDC菌株中有四株的DNA同源性水平高于60%。与CMN菌株相比，另外两株的同源性为40%，还有一株明显不同。所有七株LDC菌株的DNA与麻风分枝杆菌的DNA杂交率为0.3 - 18%，与参考棒状杆菌的杂交率为5 - 16%，与牛分枝杆菌的杂交率为5 - 12%，与豚鼠诺卡氏菌的杂交率为2 - 8%。LDC基因组的小尺寸及其与麻风分枝杆菌和CMN组生物基因组的不相关性表明了LDC的独特性。