从麻风分枝杆菌中分离出的DNA：通过光学DNA-DNA重缔合测定的基因组大小、碱基比例以及与其他相关细菌的同源性。
DNA isolated from Mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical DNA-DNA reassociation.
作者信息
Imaeda T, Kirchheimer W F, Barksdale L
出版信息
J Bacteriol. 1982 Apr;150(1):414-7. doi: 10.1128/jb.150.1.414-417.1982.
Abstract
DNA derived from Mycobacterium leprae (grown in armadillos) was isolated, purified, and analyzed spectrophotometrically. The genome size and the guanine-plus-cytosine content of M. leprae were 1.3 x 10(9) and 55.8%, respectively. Among selected strains of mycobacterial, nocardial, and corynebacterial species, Corynebacterium sp. 2628 LB, isolated from a human leprosy patient, showed the highest DNA homology with M. leprae. Of the DNAs derived from mycobacteria, those of M. tuberculosis and M. scrofulaceum showed a comparatively high reassociation with the DNMA of M. liprae.
摘要
从犰狳体内生长的麻风分枝杆菌中提取的DNA被分离、纯化，并进行了分光光度分析。麻风分枝杆菌的基因组大小和鸟嘌呤加胞嘧啶含量分别为1.3×10⁹和55.8%。在所选的分枝杆菌、诺卡氏菌和棒状杆菌菌株中，从一名人类麻风病患者分离出的棒状杆菌2628 LB与麻风分枝杆菌的DNA同源性最高。在分枝杆菌来源的DNA中，结核分枝杆菌和瘰疬分枝杆菌的DNA与麻风分枝杆菌的DNA显示出相对较高的重结合率。
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本文引用的文献
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