Singh B N, Pramanik K
Center of Excellence in Tissue Engineering, Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, India.
Center of Excellence in Tissue Engineering, Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, India.
Tissue Cell. 2018 Dec;55:83-90. doi: 10.1016/j.tice.2018.10.003. Epub 2018 Oct 30.
Lack of potential regenerative medicine to reconstruct damaged cartilage tissue has accelerated investigation and development of potential biomaterial for cartilage tissue engineering. In this study, we fabricated micron-sized non-mulberry silk fibroin fiber (SFF) using N,N-Dimethylacetamide (DMA)/10% LiBr solution and further used to develop SFF reinforced chitosan(CH) based porous scaffold with desired pore size, porosity, swelling and structural stability. The developed scaffold was characterized for its various physico-chemical, mechanical and biological properties. The developed CH/SFF composite scaffold facilitates human mesenchymal stem cell (hMSCs) attachment, colonization and extracellular matrix deposition. Furthermore, hMSCs shows significantly higher sulfated glycosaminoglycan deposition over CH/SFF in comparison to pure chitosan scaffold (control). Immunocytochemistry studies have shown enhanced expression of collagen type II and aggrecan by hMSCs over composite scaffold than chitosan scaffold. Thus, non-mulberry silk fibroin fiber reinforced chitosan based scaffold might be suitable scaffold that can act as a potential artificial matrix for cartilage tissue engineering.
缺乏用于重建受损软骨组织的潜在再生医学方法，加速了对软骨组织工程潜在生物材料的研究与开发。在本研究中，我们使用N，N - 二甲基乙酰胺（DMA）/10% 溴化锂溶液制备了微米级非桑蚕丝素蛋白纤维（SFF），并进一步用于开发具有所需孔径、孔隙率、溶胀性和结构稳定性的SFF增强壳聚糖（CH）基多孔支架。对所开发的支架进行了各种物理化学、力学和生物学性能表征。所开发的CH/SFF复合支架促进人间充质干细胞（hMSCs）的附着、定植和细胞外基质沉积。此外，与纯壳聚糖支架（对照）相比，hMSCs在CH/SFF上显示出明显更高的硫酸化糖胺聚糖沉积。免疫细胞化学研究表明，与壳聚糖支架相比，hMSCs在复合支架上II型胶原蛋白和聚集蛋白聚糖的表达增强。因此，非桑蚕丝素蛋白纤维增强壳聚糖基支架可能是一种合适的支架，可作为软骨组织工程的潜在人工基质。
