AP-HP, Service de Cytogénétique et Biologie de la Reproduction-CECOS, Hôpital Jean Verdier, Bondy, France; Université, Paris XIII, Bobigny, France.
AP-HP, Service de Cytogénétique et Biologie de la Reproduction-CECOS, Hôpital Jean Verdier, Bondy, France.
Fertil Steril. 2018 Dec;110(7):1290-1297. doi: 10.1016/j.fertnstert.2018.07.1153.
To determine whether post-warming culture duration (1 hour vs. 18 hours) influences implantation rates (IRs) of good-quality blastocysts (GQB) in a good-prognosis population.
Prospective interventional randomized study.
University hospital.
PATIENT(S): One hundred sixty-two GQB transfers.
INTERVENTION(S): Patients' vitrified blastocysts were randomly allocated to group A, warming on the day before transfer (n = 81), or B, warming on the day of transfer (n = 81).
MAIN OUTCOME MEASURE(S): IR, live birth rate, reexpansion degree, and quality after warming and immediately before transfer.
RESULT(S): Quality of the warmed and transferred blastocysts was similar (respectively, 39.1% and 32.7% top quality [≥B4AA/AB/BA] in group A vs. 41.7 and 42.2% in group B). In group A, 14 of 102 blastocysts (12.2%) appeared to be unsuitable for transfer, versus only 1 of 103 (0.9%) in group B, thus leading to an additional warming. As expected, reexpansion degree just before transfer was higher in group A (0.90 vs. 0.70). Likewise, the proportion of hatched blastocysts before transfer was higher after a longer culture period (38.6% in group A vs. 12.7% in group B). IRs were similar (38.0% in group A vs. 36% in group B), as were live birth rates (35.8% in group A vs. 34.6% in group B).
CONCLUSION(S): IRs were not different, whatever the duration of post-warming culture of GQB. Both warming strategies could be applied to good-prognosis patients to optimize the laboratory workflow without any detrimental effect.
确定在预后良好的人群中，解冻后培养时间（1 小时与 18 小时）是否会影响优质囊胚（GQB）的着床率（IR）。
前瞻性干预性随机研究。
大学医院。
162 个 GQB 移植。
将患者的冷冻囊胚随机分配至 A 组（解冻日提前，n=81）或 B 组（解冻日当天，n=81）。
IR、活产率、解冻后扩张度，以及解冻和转移前的胚胎质量。
解冻和转移的囊胚质量相似（A 组分别有 14/102 个（12.2%）囊胚质量较差[≥B4AA/AB/BA]，B 组有 1/103 个（0.9%）囊胚质量较差），因此需要额外解冻。正如预期的那样，A 组在转移前的扩张度更高（0.90 比 0.70）。同样，在较长的培养时间后，孵化囊胚的比例更高（A 组 38.6%，B 组 12.7%）。IR 相似（A 组 38.0%，B 组 36%），活产率也相似（A 组 35.8%，B 组 34.6%）。
无论 GQB 解冻后培养时间长短如何，IR 均无差异。这两种解冻策略都可以应用于预后良好的患者，以优化实验室工作流程，而不会产生任何不利影响。
