College of Marine Science & Technology, Zhejiang Ocean University, Zhoushan 316022, PR China.
College of Marine Science & Technology, Zhejiang Ocean University, Zhoushan 316022, PR China.
Mar Pollut Bull. 2018 Dec;137:267-276. doi: 10.1016/j.marpolbul.2018.10.021. Epub 2018 Oct 19.
Marine Chlorella vulgaris, an efficient hydrocarbon-degrading organism, is easily affected by light. In this study, we investigated the direct effects of different light conditions on crude oil degradation by C. vulgaris and its crude enzyme. Under 12 h illumination, the crude enzyme improved hydrocarbon removal by 39.36%, whereas the addition of the enzyme and C. vulgaris increased the degradation rate by 121.73%. Conversely, the addition of enzyme under heterotrophic condition was negatively related to oil degradation by C. vulgaris, and the degradation rate decreased from 74.32% to 48.65% and further reduced by 34.54%. The results of RNA sequencing analysis suggested that hydrocarbons removal was attributed to C. vulgaris metabolism in heterotrophic physiological state. While enhanced removal efficiency of hydrocarbons was achieved in mixotrophic physiological state due to the coupling of C. vulgaris metabolism with photocatalytic oxidation. Functional enzymes played key roles in photocatalysis and biodegradation processes.
海洋小球藻是一种高效的烃类降解生物，容易受到光照的影响。本研究探讨了不同光照条件对小球藻及其粗酶降解原油的直接影响。在 12 h 光照下，粗酶提高了 39.36%的烃类去除率，而添加酶和小球藻则将降解率提高了 121.73%。相反，在异养条件下添加酶与小球藻对油的降解呈负相关，降解率从 74.32%降至 48.65%，进一步降低了 34.54%。RNA 测序分析的结果表明，烃类的去除归因于小球藻在异养生理状态下的代谢。而在混合营养生理状态下，由于小球藻代谢与光催化氧化的耦合，提高了烃类的去除效率。功能酶在光催化和生物降解过程中发挥着关键作用。
