College of Marine Life, Ocean University of China, Qingdao, Shandong 266000, China; Key Laboratory of Watershed Sciences and Health of Zhejiang Province, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.
College of Marine Life, Ocean University of China, Qingdao, Shandong 266000, China.
Mar Pollut Bull. 2018 Dec;137:677-687. doi: 10.1016/j.marpolbul.2018.09.026. Epub 2018 Nov 15.
A microextraction method for the determination of triclosan and methyltriclosan in marine environmental samples was developed. The disperser was first serves as a preliminary extractant for analytes, then as a frozen solvent to remove impurities at -20 °C, and finally as a disperser agent in the microextraction procedure. With the extractants solidified and float on the surface of the aqueous phase at low temperature, a separation was achieved to avoided use of specialized laboratory instruments. The method was optimized using Plackett-Burman design and central composite design as follows: 146 μL octanoic acid as extractant, 793 μL acetoneas disperser, 3.0 min centrifugation and 1.1 min vortex time. The limits of detection were 0.022-0.060 μg L or μg kg and recoveries were 83.3-103.5% for TCS and MTCS in seawater, sediments and seafood. The method has excellent prospects for sample pre-treatment and trace-level analysis of triclosan and methyltriclosan in marine environmental samples.
建立了一种用于测定海洋环境样品中三氯生和甲基三氯生的微萃取方法。分散剂首先用作分析物的初步萃取剂，然后在-20°C 下用作冷冻溶剂以去除杂质，最后在微萃取过程中用作分散剂。在低温下，萃取剂凝固并漂浮在水相表面上，实现了分离，避免了使用专门的实验室仪器。该方法使用 Plackett-Burman 设计和中心复合设计进行了优化，如下所示：146 μL 辛酸作为萃取剂，793 μL 丙酮作为分散剂，离心 3.0 分钟，涡旋 1.1 分钟。在海水中、沉积物和海鲜中，TCS 和 MTCS 的检测限为 0.022-0.060μg/L 或μg/kg，回收率为 83.3-103.5%。该方法在海洋环境样品中痕量三氯生和甲基三氯生的样品预处理和痕量分析方面具有广阔的前景。
