Nutrition and Functional Food Innovation Center. Institute of Food Science and Technology CAAS, Chinese Academy of Agricultural Sciences, Beijing, China.
Nutrition and Functional Food Innovation Center. Institute of Food Science and Technology CAAS, Chinese Academy of Agricultural Sciences, Beijing, China.
Biochem Biophys Res Commun. 2019 Jan 8;508(2):494-498. doi: 10.1016/j.bbrc.2018.11.050. Epub 2018 Nov 30.
With co-treatment of potassium oxonate (PO) and xanthine sodium salt (XSS), a zebrafish larva model of acute hyperuricemia has been constructed for the first time. The results show PO 200 μM + XSS 10 μM, PO 300 μM + XSS 15 μM, and PO 400 μM + XSS 20 μM can significantly increase the level of uric acid in the zebrafish larvae (P < 0.05), the concentrations as described above can be used to construct the zebrafish larvae model of acute hyperuricemia. At the same time, treatment of allopurinol (APL, one of the hyperuricemia drugs) at 2000 μM (P < 0.001) and treatment of anserine (ASE) at 200 μM (P < 0.05) could significantly decrease the level of uric acid in the model group which received PO 200 μM + XSS 10 μM, which demonstrate that such model could offer a new robust approach for high-throughput screening of food and drugs with uric acid-lowering activity.
首次构建了一种基于黄嘌呤氧化酶抑制剂氧嗪酸钾（PO）和黄嘌呤钠盐（XSS）联合作用的斑马鱼幼鱼急性高尿酸血症模型。结果表明，200 μM PO+10 μM XSS、300 μM PO+15 μM XSS 和 400 μM PO+20 μM XSS 可显著提高斑马鱼幼鱼尿酸水平（P < 0.05），可用于构建急性高尿酸血症斑马鱼幼鱼模型。同时，2000 μM 别嘌醇（APL，一种降尿酸药物）（P < 0.001）和 200 μM 鹅肌肽（ASE）（P < 0.05）处理可显著降低 PO 200 μM+XSS 10 μM 处理组的尿酸水平，表明该模型可为具有降尿酸活性的食品和药物的高通量筛选提供一种新的有力方法。
