Salini Michael J, Wang Weifang, Mock Thomas S, Siddik Muhammad A B, Jago Matthew K, Bews Kelsey L F, Francis David S
Nutrition and Seafood Laboratory (NuSea.Lab), School of Life and Environmental Sciences, Deakin University, Queenscliff, Victoria, Australia.
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China.
Aquac Nutr. 2025 Mar 3;2025:7291857. doi: 10.1155/anu/7291857. eCollection 2025.
There is growing interest in the use of single cell proteins (SCPs) derived from methanotrophic bacteria for inclusion in aquafeed to reduce reliance on other, potentially less sustainable proteins. This two-part experiment aimed to investigate first (i) the dose effect of replacing soy protein concentrate with SCP in Australian hybrid abalone diets () and second, (ii) the potential for improved palatability of the SCP by using commercially produced liquid protein hydrolysate (PH). This was assessed in a 2 × 2 factorial experimental design. The diets were formulated to be iso-proteic (40% crude protein as fed) and iso-energetic (18 MJ kg as fed). The diets (SCP 0%, SCP 5%, SCP 10%, SCP 20%, SCP 0% + PH, and SCP 20% + PH) were fed to the abalone (~9.25 g initial weight) once daily in the evening for 94 days to apparent satiation. The growth performance and feed utilization of abalone fed with up to 10% SCP was comparable to the control; however, there was a significant reduction in most parameters at the highest inclusion of 20%. In the second experiment, there was a decline in measured protein and energy retention in the abalone fed 20% SCP, with a concomitantly lower apparent biological value. Positively, there were significant improvements in the apparent digestibility of the diet at the 20% SCP inclusion. There was no significant impact of using the PH on growth performance or feed intake. No effects were observed on the digestive enzyme activity (-amylase, lipase, and trypsin) examined among the treatments. Considering these results collectively, the maximum recommended inclusion of methanotrophic SCP meal should be 10% for hybrid abalone. Reasons for the decline in performance at the highest inclusion may be related to palatability; however, this remains to be fully confirmed.
人们越来越关注利用源自甲烷营养菌的单细胞蛋白（SCP）添加到水产饲料中，以减少对其他可能可持续性较低的蛋白质的依赖。这个分为两部分的实验旨在首先研究（i）在澳大利亚杂交鲍鱼饲料中用SCP替代大豆浓缩蛋白的剂量效应（），其次研究（ii）通过使用商业生产的液体蛋白水解物（PH）来提高SCP适口性的可能性。这是在一个2×2析因实验设计中进行评估的。饲料配方设计为等蛋白（投喂时粗蛋白约40%）和等能量（投喂时约18 MJ kg）。这些饲料（SCP 0%、SCP 5%、SCP 10%、SCP 20%、SCP 0% + PH和SCP 20% + PH）每天傍晚投喂一次给鲍鱼（初始体重约9.25 g），持续94天直至明显饱食。投喂高达10% SCP的鲍鱼的生长性能和饲料利用率与对照组相当；然而，在最高添加量20%时，大多数参数显著下降。在第二个实验中，投喂20% SCP的鲍鱼中测得的蛋白质和能量保留率下降，同时表观生物学价值较低。积极的一面是，在添加20% SCP时，饲料的表观消化率有显著提高。使用PH对生长性能或采食量没有显著影响。在各处理之间未观察到对所检测的消化酶活性（淀粉酶、脂肪酶和胰蛋白酶）有影响。综合考虑这些结果，杂交鲍鱼中甲烷营养型SCP粉的最大推荐添加量应为10%。在最高添加量时性能下降的原因可能与适口性有关；然而，这仍有待充分证实。
