Ricci R, Perazzi A, Badon T, Bedin S, Iacopetti I
Department of Animal Medicine, Production and Health, University of Padua, 16 Viale dell'Università, 35020 Legnaro, Italy.
Department of Animal Medicine, Production and Health, University of Padua, 16 Viale dell'Università, 35020 Legnaro, Italy.
Vet J. 2018 Dec;242:44-47. doi: 10.1016/j.tvjl.2018.10.006. Epub 2018 Oct 23.
Research or diagnostic conditions may require the storage of salivary samples for long periods before analysis is processed. The aim of this study was to evaluate the stability of canine salivary α-amylase, lysozyme, lactate dehydrogenase (LDH), and total calcium and phosphorus after storage for 1, 3 and 6 months at -20°C. Available saliva samples were 75 immediately after collection (T0) and 46 at 1 month (T1), 31 at 3 months (T3) and 18 at 6 months (T6) of storage, according to the number of aliquots collected from each saliva sample. Compared to T0, LDH declined by 92.3% after 1 month of storage (P<0.001), whereas lysozyme concentration significantly decreased as storage time increased (-29.3%, -43.4% and -59.1% at T1, T3, and T6 respectively, P<0.001). Amylase maintained basically the same concentration for the entire experimental period, whereas total calcium and phosphorus concentration decreased over time (calcium, P<0.001). Assessing the long-term stability of canine salivary analytes stored at -20°C may have important implications in diagnosis and research.
在进行分析之前，研究或诊断可能需要长期保存唾液样本。本研究的目的是评估犬唾液α-淀粉酶、溶菌酶、乳酸脱氢酶（LDH）以及总钙和总磷在-20°C下储存1、3和6个月后的稳定性。根据从每个唾液样本中收集的等分试样数量，收集后立即获得的可用唾液样本为75份（T0），储存1个月时为46份（T1），3个月时为31份（T3），6个月时为18份（T6）。与T0相比，储存1个月后LDH下降了92.3%（P<0.001），而溶菌酶浓度随着储存时间的增加而显著降低（T1、T3和T6时分别下降-29.3%、-43.4%和-59.1%，P<0.001）。淀粉酶在整个实验期间基本保持相同浓度，而总钙和总磷浓度随时间下降（钙，P<0.001）。评估储存在-20°C下的犬唾液分析物的长期稳定性可能对诊断和研究具有重要意义。
