Brément T, Cossec C, Roux C, Knol A C, Dréno B, Khammari A, Bourdeau P, Bruet V
Dermatology/Parasitology/Mycology Unit, School of Veterinary Medicine-ONIRIS, University of Nantes, CS 40706, Nantes, France.
NP3 Unit, School of Veterinary Medicine-ONIRIS, University of Nantes, CS 40706, Nantes 44307, France.
J Comp Pathol. 2019 Feb;167:82-90. doi: 10.1016/j.jcpa.2018.10.179. Epub 2018 Nov 28.
Adipokines are biologically active cytokines that are mainly produced in adipose tissue. There is evidence, in man and mice, that some adipokines may be secreted in other tissues including the vascular endothelium, epithelia and sebaceous glands. Moreover, modified serum levels of adipokines have been detected in people with acne vulgaris or psoriasis; it is suspected that adipokines could contribute to local and systemic inflammatory conditions. We aimed to evaluate the expression of three adipokines (i.e. leptin, adiponectin and resistin) in normal canine skin. Formalin-fixed, paraffin wax-embedded punch biopsy samples were obtained from the sparsely-haired skin of the caudal ventral abdomen of a single clinically healthy dog with no history of skin disease. Immunohistochemistry was applied, using rabbit polyclonal primary antibodies specific for leptin, adiponectin and resistin. Adipokines were not expressed in normal canine dermis or hypodermis. In contrast, they were detected in the keratinocytes of all epidermal layers and hair follicle segments, sebocytes, apocrine gland cells and in the vascular endothelium. This is the first report on the expression of adipokines in normal canine skin, a first step in studying their role in the skin physiology and inflammatory skin diseases of dogs.
脂肪因子是主要在脂肪组织中产生的生物活性细胞因子。在人类和小鼠中，有证据表明一些脂肪因子可能在包括血管内皮、上皮和皮脂腺在内的其他组织中分泌。此外，在寻常痤疮或银屑病患者中检测到脂肪因子的血清水平发生了改变；人们怀疑脂肪因子可能导致局部和全身炎症状态。我们旨在评估三种脂肪因子（即瘦素、脂联素和抵抗素）在正常犬皮肤中的表达。从一只临床上健康且无皮肤病史的犬的尾腹侧无毛皮肤获取福尔马林固定、石蜡包埋的打孔活检样本。使用针对瘦素、脂联素和抵抗素的兔多克隆一抗进行免疫组织化学检测。脂肪因子在正常犬的真皮或皮下组织中不表达。相反，在所有表皮层和毛囊段的角质形成细胞、皮脂腺细胞、顶泌汗腺细胞以及血管内皮中检测到了它们。这是关于脂肪因子在正常犬皮肤中表达的首次报道，是研究它们在犬皮肤生理学和炎症性皮肤病中作用的第一步。
