奥罗普切正布尼亚病毒：全长基因组的遗传特征分析及用于鉴别自然重组的分子方法的开发。
Oropouche orthobunyavirus: Genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments.
机构信息
Center for Technological Innovations, Evandro Chagas Institute, Ministry of Health, Ananindeua, Pará, Brazil; Center for Biodefense and Emerging Infectious Diseases, Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
Virology Research Center, School of Medicine of Ribeirao Preto of University of São Paulo, Ribeirao Preto, São Paulo, Brazil; MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
出版信息
Infect Genet Evol. 2019 Mar;68:16-22. doi: 10.1016/j.meegid.2018.11.020. Epub 2018 Nov 29.
Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences (S-, M- and L-RNA) of 35 OROV isolates from Brazil. Here, we classified 20 strains in genotype I from Pará and Maranhão states, nine as genotype II from Pará and Rondônia states confirmed, four classified into genotype III from Acre, Maranhão, Minas Gerais and Rondônia states and two genotype IV from Amazonas State. Also, we did not observe reassortment events involving the OROV isolates. In addition, we developed novel RT-PCR tools to identify reassortment events among OROV strains. These data will be useful to better understand the molecular epidemiology and diagnostic of OROV infections.
奥罗普切病毒（OROV）对亚马逊地区的公共卫生有重大影响。这种虫媒病毒是巴西最常见的发热疾病病原体之一，自 20 世纪 60 年代以来引发了数次疫情。在这项研究中，我们对来自巴西的 35 个 OROV 分离株的全长编码序列（S-、M-和 L-RNA）进行了测序和特征分析。在此，我们将来自帕拉州和马拉尼昂州的 20 株划分为基因型 I，来自帕拉州和朗多尼亚州的 9 株确认为基因型 II，来自阿克里州、马拉尼昂州、米纳斯吉拉斯州和朗多尼亚州的 4 株划分为基因型 III，来自亚马孙州的 2 株划分为基因型 IV。此外，我们没有观察到涉及 OROV 分离株的重配事件。此外，我们开发了新的 RT-PCR 工具来鉴定 OROV 株之间的重配事件。这些数据将有助于更好地了解 OROV 感染的分子流行病学和诊断。
Zotero 插件