与奥罗普切病毒感染相关的不良妊娠结局病例系列

Case Series of Adverse Pregnancy Outcomes Associated with Oropouche Virus Infection.

作者信息

Medeiros Daniele Barbosa de Almeida, Quaresma Juarez Antônio Simões, Azevedo Raimunda do Socorro da Silva, Cruz Ana Cecilia Ribeiro, Silva Sandro Patroca da, Martins Filho Arnaldo Jorge, Nunes Bruno Tardelli Diniz, Pinheiro Lucas Rafael Santana, Sousa Jorge Rodrigues de, Chiang Jannifer Oliveira, Martins Lívia Carício, Oliveira Consuelo Silva, Prazeres Ivy Tissuya Essashika, Henriques Daniele Feitas, Oliveira Camille Ferreira, Carvalho Valéria Lima, Morais Clarice Neuenschwander Lins, Acioli-Santos Bartolomeu, Silva Keilla Maria Paze, Falcão Diego Arruda, Costa Mayara Matias de Oliveira Marques, Bezerra Eduardo Augusto Duque, Rio Ana Márcia Drechsler, Cardoso Neijla Cristina Vieira, Silva Juliana Carla Serafim da, Ramos Simone Gurmão, Maranhão Erika Cavalcante, Lima José Lancart de, Vasconcelos Pedro Fernando da Costa, Ishigami Bruno Issao Matos, Casseb Lívia Medeiros Neves

机构信息

Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n-Levilândia, Ananindeua 67030-000, Pará, Brazil.

Department of Entomology, Aggeu Magalhães Institute (IAM), Recife 50740-465, Pernambuco, Brazil.

出版信息

Viruses. 2025 Jun 5;17(6):816. doi: 10.3390/v17060816.

DOI:10.3390/v17060816

PMID:40573407

Abstract

The Oropouche virus (OROV) is an arbovirus (Peribunyaviridae: Orthobunyavirus) that traditionally causes febrile outbreaks in Latin America's Amazon region. Previously, OROV was not associated with severe pregnancy outcomes. During the 2022-2024 outbreak in Brazil, OROV expanded geographically, revealing links to adverse pregnancy outcomes. This study describes six cases with varied fetal outcomes, including miscarriage, antepartum, intrauterine fetal demise (IFD), and normal development, correlating with maternal symptoms but not symptom severity. Vertical transmission was confirmed by detecting OROV through RT-qPCR, ELISA, and immunohistochemistry in fetal tissues. Genome sequencing from an IFD case identified a novel reassortment pattern reported in the 2022-2024 outbreak. Severe encephalomalacia, meningoencephalitis, vascular compromise, and multi-organ damage were evident, underscoring the significant risk OROV poses to fetal development and emphasizing the need for further investigation.

摘要

奥罗普切病毒（OROV）是一种虫媒病毒（布尼亚病毒科：正布尼亚病毒属），传统上在拉丁美洲的亚马逊地区引发发热疫情。此前，OROV与严重的妊娠结局并无关联。在巴西2022 - 2024年的疫情期间，OROV的地理分布范围扩大，显示出与不良妊娠结局的关联。本研究描述了6例胎儿结局各异的病例，包括流产、产前、宫内胎儿死亡（IFD）和正常发育，这些结局与母亲的症状相关，但与症状严重程度无关。通过在胎儿组织中进行逆转录定量聚合酶链反应（RT-qPCR）、酶联免疫吸附测定（ELISA）和免疫组织化学检测OROV，证实了垂直传播。对一例IFD病例进行的基因组测序确定了一种在2022 - 2024年疫情中报告的新型重配模式。严重的脑软化、脑膜脑炎、血管损伤和多器官损伤明显，凸显了OROV对胎儿发育构成的重大风险，并强调了进一步调查的必要性。