Fujii Makiko, Kato Kiku, Imai Miko, Kuwabara Hiroki, Awano Minori, Hashizaki Kaname, Taguchi Hiroyuki
School of Pharmacy, Nihon University.
Biol Pharm Bull. 2019 Feb 1;42(2):295-298. doi: 10.1248/bpb.b18-00749. Epub 2018 Dec 1.
Surface free energy (SFE) is an important factor for evaluation of wettability or adhesion. Thus, the SFE of a Yucatan micropig (YMP) skin and a hairless mouse (HM) skin, which are commonly used in skin permeation studies instead of human skin, were compared with the human skin. Contact angles of water and 1-bromo naphthalene to skin were measured and the SFE was calculated using the Owens-Wendt equation. The SFE of the human abdominal skin was 40 mN/m and its polar component σ was as low as 2 mN/m, which was similar to that of the low sebum skin reported previously. In the case of the YMP skin, σ was high on the surface but similar to that obtained after the skin was tape-stripped twice. The HM skin showed similar SFE as that of the human skin. When the surfactant was applied on the skin, wiped, and dried, the remaining surfactant increased the SFE in σ; however, the original SFE was obtained after rinsing with water. The YMP skin and HM skin is similar to the human abdominal skin with a low sebum level. Thus, they are also good skin models for studying wettability or adhesion of a substance.
表面自由能（SFE）是评估润湿性或粘附性的一个重要因素。因此，将常用于皮肤渗透研究而非人体皮肤的尤卡坦小型猪（YMP）皮肤和无毛小鼠（HM）皮肤的表面自由能与人体皮肤进行了比较。测量了水和1-溴萘与皮肤的接触角，并使用欧文斯-温德特方程计算表面自由能。人体腹部皮肤的表面自由能为40 mN/m，其极性成分σ低至2 mN/m，这与先前报道的低皮脂皮肤相似。对于YMP皮肤，其表面的σ较高，但与皮肤经两次胶带剥离后得到的结果相似。HM皮肤的表面自由能与人体皮肤相似。当表面活性剂涂抹在皮肤上、擦拭并干燥后，残留的表面活性剂会增加σ中的表面自由能；然而，用水冲洗后可得到原始的表面自由能。YMP皮肤和HM皮肤与皮脂水平较低的人体腹部皮肤相似。因此，它们也是研究物质润湿性或粘附性的良好皮肤模型。
