Liu Ruize, Deng Dadong, Liu Xiangdong, Xiao Yujing, Huang Ji, Wang Feiyu, Li Xinyun, Yu Mei
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Mamm Genome. 2019 Feb;30(1-2):34-41. doi: 10.1007/s00335-018-9788-x. Epub 2018 Dec 1.
Increasing evidence suggests that miRNA binding-site polymorphism in the 3'-untranslated region (3'UTR) of a target gene could affect that gene's expression, and can be associated with a variety of complex traits. In this study, we find that miR-18a and cell division cycle 42 (CDC42) mRNA, whose expression was inversely correlated, are differentially expressed in porcine placentas during critical stages of placental development. rs55618224 (T>C), a SNP in the 3'UTR region of CDC42 that is perfectly complementary to the miR-18a seed could influence miR-18a-related regulation of CDC42 gene by altering their binding affinity. In addition, CDC42 mRNA was found to have higher expression level in the homozygous TT placentas as compared to those homozygous CC placentas in pigs. Furthermore, we identified a significant association between rs55618224 and total number born per litter. These results suggest the miR-18a binding-site polymorphism in CDC42 3'UTR may impact litter size by regulation of CDC42 gene in porcine placentas.
越来越多的证据表明，靶基因3'-非翻译区（3'UTR）中的miRNA结合位点多态性可能影响该基因的表达，并可能与多种复杂性状相关。在本研究中，我们发现miR-18a与细胞分裂周期42（CDC42）mRNA的表达呈负相关，且在胎盘发育关键阶段的猪胎盘中差异表达。rs55618224（T>C）是CDC42 3'UTR区域中的一个单核苷酸多态性，与miR-18a种子序列完全互补，它可能通过改变结合亲和力来影响miR-18a相关的CDC42基因调控。此外，发现猪中纯合TT胎盘的CDC42 mRNA表达水平高于纯合CC胎盘。此外，我们确定了rs55618224与每窝产仔总数之间存在显著关联。这些结果表明，CDC42 3'UTR中的miR-18a结合位点多态性可能通过调控猪胎盘内的CDC42基因来影响产仔数。
