Yang Hui, Zou Pu, Chen Jingyuan, Shi Gaoxing, Wu Chenlu, Wang Mi, Zhou Qin, Zhou Shenghua
Department of Cardiology, The Second Xiangya Hospital, Central South University.
Department of General Surgery, The Second Xiangya Hospital, Central South University;
J Vis Exp. 2018 Nov 18(141). doi: 10.3791/58499.
Blood collection with enough blood volume is essential in animal experiments. Blood collection from the tail vein of rats is popular and less stressful compared to other more aggressive methods such as retro-orbital plexus sample collection. However, this blood collection method is sometimes limited by an unsatisfactory success rate. Here, we introduce a method for blood collection through the subclavian vein puncture. The subclavian vein is located just under the clavicle and this vein is large enough to fulfill the volume requirements of blood collection. Our results show that this method is safe and applicable for blood collection sampling with the required blood volume. Blood collection through the subclavian vein puncture could serve as an alternative blood collection method in case of failed tail vein blood sampling in rats.
在动物实验中，采集足够血量的血液至关重要。与其他更具侵入性的方法（如眶后丛采血）相比，从大鼠尾静脉采血很常见且压力较小。然而，这种采血方法有时会因成功率不理想而受到限制。在此，我们介绍一种通过锁骨下静脉穿刺采血的方法。锁骨下静脉位于锁骨下方，该静脉足够粗大，能够满足采血的血量要求。我们的结果表明，这种方法安全且适用于采集所需血量的血液样本。在大鼠尾静脉采血失败的情况下，通过锁骨下静脉穿刺采血可作为一种替代的采血方法。
