Guangdong Lewwin Pharmaceutical Research Institute Co., Ltd., Guangdong Provincial Key Laboratory of Drug Non-Clinical Evaluation and Research, TCM Non-clinic Evaluation Branch of National Engineering Research Center for Modernization of Traditional Chinese Medicine, Guangdong Engineering Research Center for Innovative Drug Evaluation and Research.
Department of Biomedical Engineering, School of Basic Medical Sciences, the Sixth Affiliated Hospital of Guangzhou Medical University;
J Vis Exp. 2022 Feb 9(180). doi: 10.3791/63439.
Rats are widely used in pharmacokinetics (PK) and toxicokinetic (TK) studies that need to collect a certain amount of blood at specific time points to detect drug exposure. The rat blood sampling method determines the quality of the plasma and further affects the precision of the test results. The subclavian vein blood collection method described in this protocol collects blood samples repeatedly in the conscious state of animals to meet the needs of PK and TK tests. The skills of restraint handling and appropriate procedure of needle incision ensure the success rate of blood collection. It is easy to operate while ensuring the quality of plasma and at the same time catering to animal welfare. However, this method requires skilled operation, and an improper one may cause animal weakness, pain, lameness, and even mortality. The current method has been used in the testing facility for a 4-week oral toxicity study in Sprague Dawley (SD) rats with TK. The maximum amount of blood collected within 24 h did not exceed 20% of the animal's total blood. The animals' body weight was more than 200 g for males and females. The data showed that the animals' bodyweight increased steadily every week, and the clinical observation was normal after repetitive sample collection.
大鼠被广泛用于药代动力学（PK）和毒代动力学（TK）研究，需要在特定时间点采集一定量的血液以检测药物暴露。大鼠采血方法决定了血浆的质量，进而影响试验结果的精密度。本方案中描述的锁骨下静脉采血方法在动物清醒状态下重复采集血样，以满足 PK 和 TK 试验的需求。熟练的约束处理和适当的切口程序技能确保了采血的成功率。该方法操作简单，既能保证血浆质量，又能满足动物福利的要求。然而，这种方法需要熟练的操作，不恰当的操作可能会导致动物虚弱、疼痛、跛行，甚至死亡。目前该方法已用于 SD 大鼠为期 4 周的口服毒性研究的 TK 测试中。在 24 小时内采集的最大血量不超过动物总血量的 20%。雄性和雌性大鼠的体重均超过 200g。数据显示，动物体重每周稳步增加，重复采样后临床观察正常。
