Aravind Aravindakshanpillai P Anu, Pandey Renu, Kumar Brijesh, Asha Kumarapillai R T, Rameshkumar Koranappallil B
Nat Prod Commun. 2016 Dec;11(12):1839-1842.
Qualitative screening of multiclass secondary metabolites present in the fruits, leaves and stem bark extracts of Garcinia travancorica was carried out using HPLC-QTOF-MS analysis. Twenty-three compounds were identified in the fruits, leaves and stem bark; including two acids (hydroxycitric acid and hydroxycitric acid lactone), eight biflavonoids (morelloflavone, GB-1, GB-la, GB-2, GB-2a, fukugiside, xanthochymusside and GB-la glucoside), nine xanthones (α-mangostin, γ-mangostin, 1,5-dihydroxy-3-methoxyxanthone, garciniaxanthone E, 4-(I,1-dimethylprop-2-enyl)-1,3,5,8-tetrahydroxy-xanthone, garcinone A, garcinone B, garcinone C and polyanxanthone C) and four polyisoprenylated benzophenones (gambogenone, aristophenone A, garcinol and garciyunnanin A). Cytotoxicity studies of the major biflavonoid fukugiside reported from G. travancorica leaves revealed a dose-dependent cancer cell growth inhibition in A431 and HeLa cells. The antiproliferative effect appears to be due to the ability of fukugiside to induce S-phase arrest and apoptotic cell death. In HeLa cells, fukugiside reduced the expression of MAPKp38 by 26.1% compared with untreated control.
采用高效液相色谱-四极杆飞行时间质谱（HPLC-QTOF-MS）分析法，对多花山竹子果实、叶片和茎皮提取物中存在的多类次生代谢产物进行了定性筛选。在果实、叶片和茎皮中鉴定出了23种化合物；包括两种酸（羟基柠檬酸和羟基柠檬酸内酯）、八种双黄酮（莫雷黄酮、GB-1、GB-1a、GB-2、GB-2a、福木苷、山竹黄酮苷、GB-1a葡萄糖苷）、九种呫吨酮（α-山竹黄酮、γ-山竹黄酮、1,5-二羟基-3-甲氧基呫吨酮、藤黄呫吨酮E、4-(I,1-二甲基丙-2-烯基)-1,3,5,8-四羟基呫吨酮、藤黄酮A、藤黄酮B、藤黄酮C和多聚呫吨酮C）以及四种多异戊烯基化二苯甲酮（藤黄双黄酮、阿里斯托芬酮A、藤黄醇和藤黄云南素A）。对多花山竹子叶片中报道的主要双黄酮福木苷进行的细胞毒性研究表明，其对A431和HeLa细胞的癌细胞生长具有剂量依赖性抑制作用。这种抗增殖作用似乎是由于福木苷能够诱导S期阻滞和凋亡性细胞死亡。在HeLa细胞中，与未处理的对照相比，福木苷使丝裂原活化蛋白激酶p38（MAPKp38）的表达降低了26.1%。
