Aravind A P Anu, Asha K R T, Rameshkumar K B
a Phytochemistry and Phytopharmacology Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode , Thiruvananthapuram 695562 , Kerala , India.
b Govt. Arts College , Paramakudy , Tamilnadu , India.
Nat Prod Res. 2016;30(2):232-6. doi: 10.1080/14786419.2015.1043551. Epub 2015 May 18.
Phytochemical analysis of the leaves of Garcinia travancorica, a hitherto uninvestigated endemic species to the Western Ghats of south India, resulted in isolation and characterisation of the polyisoprenylated benzophenones 7-epi-nemorosone (1) and garcinol (2) along with biflavonoids GB-1a (3), GB-1 (4), GB-2 (5), morelloflavone (6) and morelloflavone-7″-O-β-D-glycoside or fukugiside (7). The compounds were identified using various spectroscopic techniques, mainly through NMR and MS. The methanol extract and the biflavonoids 3, 4, 5 and 7 showed potential in vitro antioxidant activities. The IC50 value of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of compound 7 was 8.34 ± 2.12 μg/mL, comparable to that of standard ascorbic acid (3.2 ± 0.50 μg/mL). In the superoxide radical scavenging assay, compound 7 gave IC50 value of 6.95 ± 1.33 μg/mL close to standard ascorbic acid with IC50 value of 5.8 ± 0.25 μg/mL. Validated HPTLC estimation revealed G. travancorica as a rich source of morelloflavone-7″-O-β-D-glycoside (7.12% dry wt. leaves).
对印度南部西高止山脉一种此前未被研究的特有物种——特拉凡哥尔藤黄（Garcinia travancorica）的叶子进行植物化学分析，结果分离并鉴定出了多异戊烯基化二苯甲酮7-表-降香酮（1）和藤黄醇（2），以及双黄酮GB-1a（3）、GB-1（4）、GB-2（5）、桑橙素（6）和桑橙素-7″-O-β-D-糖苷或福木苷（7）。这些化合物通过各种光谱技术进行鉴定，主要是通过核磁共振（NMR）和质谱（MS）。甲醇提取物以及双黄酮3、4、5和7表现出潜在的体外抗氧化活性。化合物7对2,2-二苯基-1-苦基肼（DPPH）自由基的清除活性的IC50值为8.34±2.12μg/mL，与标准抗坏血酸（3.2±0.50μg/mL）相当。在超氧阴离子自由基清除试验中，化合物7的IC50值为6.95±1.33μg/mL，接近标准抗坏血酸的IC50值5.8±0.25μg/mL。经过验证的高效薄层色谱（HPTLC）测定表明，特拉凡哥尔藤黄是桑橙素-7″-O-β-D-糖苷的丰富来源（干叶重量的7.12%）。
