Schinkel A H, Groot Koerkamp M J, Teunissen A W, Tabak H F
Laboratory of Biochemistry, University of Amsterdam, The Netherlands.
Nucleic Acids Res. 1988 Oct 11;16(19):9147-63. doi: 10.1093/nar/16.19.9147.
Yeast mitochondrial RNA polymerase can bind specifically to promoter-containing DNA fragments in vitro as detected by DNAse I or methidiumpropyl-EDTA. Fe(II) protection assays and gel retardation experiments. Retardation of RNA polymerase-DNA complexes was most pronounced when the promoter was located in the middle of a DNA fragment and was diminished when RNA polymerase was bound near one of the ends. This indicates that upon RNA polymerase-binding the DNA undergoes a conformational change which is most likely a bend. The degree of introduced bending correlated with the efficiency of transcription and promoter-binding in a series of promoter mutants, suggesting that bending is a functional event during promoter utilisation.
