Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China.
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, 100193, China.
Sci Rep. 2018 Dec 4;8(1):17630. doi: 10.1038/s41598-018-35945-9.
Mercury is considered to be "a global pollutant" and raises concern worldwide. Once mercury enters the body, it will be distributed all over the body but will accumulate in the brain, kidney and liver. To date, no substance originating from edible fungi capable of adsorbing mercury has been reported. We found that the mushroom Grifola frondosa exhibited mercury adsorption capacity. A polysaccharide-peptide (GFPP), displaying the unique N-terminal amino acid sequence of APPGMHQKQQ and 7 partial sequences with high reliability obtained by LC-MS/MS, was isolated by hot-water extraction of its fruiting bodies followed by ion exchange chromatography and gel filtration chromatography. Two rat models were employed to determine the dose and the duration of HgCl treatment (given by acute administration or continuous treatment) to test if G. frondosa could promote mercury elimination. For rats subjected to acute treatment with HgCl, both GFPP and G. frondosa fruiting bodies (GFFF) could accelerate the decline of blood mercury level, which fell precipitously by 50% on the second day. GFPP and GFFF also promoted elimination of the burden of mercury in the liver and kidneys. For rats receiving continuous HgCl treatment, G. frondosa prevented the progressive increase of blood mercury level, and kept the blood mercury level within a relatively stable range.
汞被认为是“全球性污染物”，引起了全世界的关注。一旦汞进入体内，它将分布在全身，但会在大脑、肾脏和肝脏中积累。迄今为止，尚未报道有任何源自可吸附汞的食用真菌的物质。我们发现，灰树花具有吸附汞的能力。一种多糖肽（GFPP），通过热水提取其子实体，然后通过离子交换色谱和凝胶过滤色谱分离，显示出 APPGMHQKQQ 的独特 N 端氨基酸序列和通过 LC-MS/MS 获得的 7 个具有高可信度的部分序列。采用两种大鼠模型来确定 HgCl 处理的剂量和时间（通过急性给药或连续处理），以测试灰树花是否可以促进汞的消除。对于接受 HgCl 急性处理的大鼠，GFPP 和灰树花子实体（GFFF）都可以加速血液中汞水平的下降，第二天下降了 50%。GFPP 和 GFFF 还促进了肝脏和肾脏中汞负担的消除。对于接受连续 HgCl 处理的大鼠，灰树花阻止了血液中汞水平的逐渐升高，并使血液中汞水平保持在相对稳定的范围内。
