Departamento de Química, Universidade Federal de Goiás, Regional Catalão, 75704-020 Catalão, Brazil.
Departamento de Química, Universidade Federal de Goiás, Regional Catalão, 75704-020 Catalão, Brazil; Laboratório de Ressonância Magnética Nuclear, Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, 74001-970 Goiânia, Brazil.
Carbohydr Polym. 2018 May 1;187:110-117. doi: 10.1016/j.carbpol.2018.01.080. Epub 2018 Jan 31.
An unusual heteropolysaccharide was isolated from the fruiting bodies of the medicinal mushroom Grifola frondosa, via successive cold aqueous extraction, followed by fractionation through freeze-thawing, precipitation with Fehling solution and dialysis using a membrane with a size exclusion cut-off of 500 kDa. Its chemical structure was determined based on total acid hydrolysis, methylation analysis and NMR studies. The mannofucogalactan had a molar mass of 15.9 × 10 g mol, which was determinate by HPSEC-MALLS. This heteropolymer showed to have a main chain of (1 → 6)-linked α-d-Galp partially substituted at O-2 by 3-O-α-d-mannopyranosyl-α-l-fucopyranosyl groups and in a minor proportion with α-l-Fucp single-unit side chains. Moreover, the presence of 3-O-Me-Galp units could also be observed in the main chain of the G. frondosa mannofucogalactan.
从药用蘑菇灰树花的子实体中，通过连续的冷水性提取，接着通过冻融分级、Fehling 溶液沉淀和使用截留分子量为 500 kDa 的膜进行透析，分离得到一种不寻常的杂多糖。基于完全酸水解、甲基化分析和 NMR 研究确定了其化学结构。甘露葡半乳糖聚糖的摩尔质量为 15.9×10g/mol，通过 HPSEC-MALLS 确定。该杂聚物的主链为（1→6）-连接的α-d-Galp，部分在 O-2 位被 3-O-α-d-甘露吡喃基-α-l-岩藻吡喃基取代，并且在较小程度上带有α-l-Fucp 单单元侧链。此外，在灰树花甘露葡半乳糖聚糖的主链中也可以观察到 3-O-Me-Galp 单元的存在。
