Mubin Nida, Umar Mohd Saad, Zubair Swaleha, Owais Mohammad
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.
Department of Computer Science, Aligarh Muslim University, Aligarh, India.
Front Microbiol. 2018 Nov 20;9:2469. doi: 10.3389/fmicb.2018.02469. eCollection 2018.
In the present study, we investigated potential of chitosan-based nanoparticles (CNPs) to deliver loaded therapeutic molecules to pathogen harboring macrophages. We fabricated stable CNPs employing ionic cross-linking method and evaluated their potential to target RAW 264.7 cells. The physicochemical characterization of as-synthesized CNPs was determined using electron microscopy, infrared microscopy and zeta potential measurement. Next, cellular uptake and intracellular localization studies of CNPs were followed in living RAW264.7 cells using confocal microscopy. We found that both Acr-1 loaded (CNP-A) and 4-SO-GalNAc ligand harboring (CNP-L) chitosan nanoparticle experience increased cellular uptake by infected RAW cells. Following cellular digestion in model macrophage cell line (RAW), CNPs provide an increased immune response. Further, 4-SO-GalNAc bearing CNP-L exhibits high binding affinity as well as antibacterial efficacy toward . The data of the present study suggest that CNP-based nanoparticle offer a promising delivery strategy to target infected macrophages for prevention and eradication of intracellular pathogens such as .
在本研究中，我们研究了基于壳聚糖的纳米颗粒（CNPs）将负载的治疗性分子递送至携带病原体的巨噬细胞的潜力。我们采用离子交联法制备了稳定的CNPs，并评估了它们靶向RAW 264.7细胞的潜力。使用电子显微镜、红外显微镜和zeta电位测量对合成的CNPs进行了物理化学表征。接下来，使用共聚焦显微镜在活的RAW264.7细胞中进行了CNPs的细胞摄取和细胞内定位研究。我们发现，负载Acr-1的（CNP-A）和携带4-SO-GalNAc配体的（CNP-L）壳聚糖纳米颗粒均会使受感染的RAW细胞的细胞摄取增加。在模型巨噬细胞系（RAW）中进行细胞消化后，CNPs会增强免疫反应。此外，携带4-SO-GalNAc的CNP-L对……表现出高结合亲和力以及抗菌功效。本研究的数据表明，基于CNP的纳米颗粒为靶向感染的巨噬细胞以预防和根除细胞内病原体（如……）提供了一种有前景的递送策略。
