Fauser A A, Kanz L, Spurll G M, Löhr G W
Division of Hematology, Royal Victoria Hospital, McGill University, Montreal, Canada.
Transplantation. 1988 Oct;46(4):543-8. doi: 10.1097/00007890-198810000-00015.
Megakaryocytic colony formation is dependent upon growth-stimulating activities present in human serum or plasma. Factors with diverse biological activities including megakaryocytic colony-stimulating activity (Mk-CSA) are provided by the medium of mitogen stimulated peripheral mononuclear cells or subsets of peripheral T cells. In this communication we describe the stimulatory activity of plasma collected from allogeneic and autologous bone marrow transplant recipients on the growth of megakaryocytic colonies. Mk-CSA was found to increase after transplantation as bone marrow regenerated. The stimulatory activities for CFU-M were greater in plasma from allogeneic bone marrow transplant patients receiving T cell-depleted donor marrow than in patients receiving unmodified donor marrow. Growth-promoting activities derived from mitogen-stimulated lymphocytes of T4 phenotype, a potent source of Mk-CSA, did not increase the frequency of CFU-M when cultured in plasma collected from transplant patients receiving a T cell-depleted donor marrow. However, a further increase in the number of CFU-M was observed when exogenous Mk-CSA was added to the cultures supplemented with plasma from patients receiving unmodified donor marrow. Plasma of patients who received autologous marrow displayed similar Mk-CSA activity when compared with the activity of plasma obtained from transplant recipients receiving an unmodified allogeneic donor marrow. Stimulatory activities supporting multilineage colonies (CFU-GEMM), erythroid bursts (BFU-E), and granulocytic colonies (CFU-C) derived from plasma of the three different transplant groups revealed no statistical difference with respect to the frequency of CFU-GEMM, BFU-E, or CFU-C colonies when compared with pretransplant plasma. The results suggest that MK-CSA may play an important role in the regulation of megakaryopoiesis in vivo. Moreover the data suggest the presence of humoral regulators that appear to be different with respect to the processing of the donor marrow.
巨核细胞集落形成依赖于存在于人类血清或血浆中的生长刺激活性。具有多种生物活性的因子，包括巨核细胞集落刺激活性（Mk-CSA），由有丝分裂原刺激的外周血单个核细胞或外周T细胞亚群的培养基提供。在本通讯中，我们描述了从同种异体和自体骨髓移植受者采集的血浆对巨核细胞集落生长的刺激活性。发现随着骨髓再生，移植后Mk-CSA增加。接受T细胞去除的供体骨髓的同种异体骨髓移植患者血浆中对CFU-M的刺激活性高于接受未处理供体骨髓的患者。来自T4表型有丝分裂原刺激淋巴细胞（Mk-CSA的强大来源）的生长促进活性，在从接受T细胞去除的供体骨髓的移植患者采集的血浆中培养时，并未增加CFU-M的频率。然而，当将外源性Mk-CSA添加到补充有接受未处理供体骨髓患者血浆的培养物中时，观察到CFU-M数量进一步增加。与接受未处理的同种异体供体骨髓的移植受者获得的血浆活性相比，接受自体骨髓患者的血浆显示出相似的Mk-CSA活性。来自三个不同移植组血浆的支持多谱系集落（CFU-GEMM）、红系爆式集落（BFU-E）和粒系集落（CFU-C）的刺激活性，与移植前血浆相比，在CFU-GEMM、BFU-E或CFU-C集落频率方面没有统计学差异。结果表明，MK-CSA可能在体内巨核细胞生成的调节中起重要作用。此外，数据表明存在体液调节因子，其在供体骨髓处理方面似乎有所不同。
