Grossi A, Vannucchi A M, Rafanelli D, Vannucchi L, Ferrini P R
Am J Hematol. 1987 Aug;25(4):371-6. doi: 10.1002/ajh.2830250403.
We studied the behavior in culture of megakaryocyte progenitor cells (CFU-Mk) from peripheral blood (PB) and bone marrow (BM) cells in eight patients with myeloproliferative diseases (MPD). In seven patients we observed megakaryocyte (Mk) colony formation from PB cells, which were generated in the absence of any added stimulator and which did not increase after the addition of a source of Mk-colony stimulating activity (CSA-Mk). The number of BM CFU-Mk was significantly higher in patients than in controls, and in seven out of eight patients the responsiveness to added CSA-Mk was retained. Plasma obtained from six patients did not stimulate normal donors' BM target cells to form Mk colonies. These data demonstrate an expansion of the CFU-Mk pool in MPD patients without increased plasma levels of CSA-Mk, and suggest that PB and BM CFU-Mk of MPD patients might have different kinetic properties.
我们研究了8例骨髓增殖性疾病（MPD）患者外周血（PB）和骨髓（BM）细胞中巨核细胞祖细胞（CFU-Mk）在培养中的行为。在7例患者中，我们观察到PB细胞形成巨核细胞（Mk）集落，这些集落在未添加任何刺激物的情况下产生，并且在添加Mk集落刺激活性来源（CSA-Mk）后并未增加。患者的BM CFU-Mk数量显著高于对照组，并且8例患者中有7例对添加的CSA-Mk仍有反应。从6例患者获得的血浆未刺激正常供体的BM靶细胞形成Mk集落。这些数据表明MPD患者的CFU-Mk池扩大，而血浆中CSA-Mk水平并未升高，并提示MPD患者的PB和BM CFU-Mk可能具有不同的动力学特性。
