Organic and Bioconjugate Chemistry Laboratory, Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhauri, Bhopal Bypass Road, Bhopal 462066, India.
Org Biomol Chem. 2018 Dec 12;16(48):9377-9381. doi: 10.1039/c8ob02897d.
We show that the chemoselectivity of an electrophile in protein labeling can be promiscuous. An aldehyde enables switching of chemoselectivity of an epoxide and a sulfonate ester along with an enhanced rate of reaction. The chemical technology renders single-site installation of diverse probes on a protein and delivers analytically pure tagged proteins.
我们证明了亲电试剂在蛋白质标记中的化学选择性可以是混杂的。醛能够切换环氧化物和磺酸酯的化学选择性，并提高反应速率。该化学技术可以在单个蛋白质位点上安装多种探针，并提供分析纯标记的蛋白质。
