Xu Yun, Wang Yifan, Ji Jian, Wu Hao, Pi Fuwei, Zhang Yinzhi, Sun Xiulan
a State Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Synergetic Innovation Center of Food Safety and Quality Control , Jiangnan University , Wuxi , Jiangsu , P.R. China.
b Research and development department , Guangzhou GRG Metrology &Test Co., Ltd , Guangzhou , Guangdong , P.R. China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2019 Jan;36(1):163-174. doi: 10.1080/19440049.2018.1547425. Epub 2018 Dec 5.
Our work mainly studied the elimination of zearalenone (ZEN) from corn flour by ozone treatment. We found four degradation products, after separation and identification by UPLC-ESI-QTOF-MS, named as Compound 1 (m/z 335.18), Compound 2 (m/z 351.19), Compound 3 (m/z 321.19) and Compound 4 (m/z 367.18). Then, one main transformation pathway was predicted on the basis of molecular weight and the reaction mechanism of ozone. We had also detected two of the degradation products by our modified UPLC-MS/MS method in real samples-contaminated corn flour. We screened the toxicity of the parent ZEN compound as well as the ozone induced decomposition products with BALB/c mice to evaluate the safety of our method in vivo. Therefore, aqueous ozone was demonstrated to effectively degrade ZEN and chemical structure alternations of ZEN after ozone treatment were predicted in both pure ZEN and real contaminated-corn flour samples. Considering the presence of the by-products formed, differences in the toxic effects on mice exposed to ZEN before and after the ozone treatment emphasise the significance of further study on the quality and toxicity of real food samples after ozone treatment.
我们的工作主要研究了通过臭氧处理从玉米粉中去除玉米赤霉烯酮（ZEN）。通过超高效液相色谱-电喷雾电离-四极杆飞行时间质谱（UPLC-ESI-QTOF-MS）分离和鉴定后，我们发现了四种降解产物，分别命名为化合物1（m/z 335.18）、化合物2（m/z 351.19）、化合物3（m/z 321.19）和化合物4（m/z 367.18）。然后，根据分子量和臭氧的反应机理预测了一条主要的转化途径。我们还通过改进的UPLC-MS/MS方法在实际样品——受污染的玉米粉中检测到了两种降解产物。我们用BALB/c小鼠筛选了母体ZEN化合物以及臭氧诱导的分解产物的毒性，以评估我们的方法在体内的安全性。因此，在纯ZEN和实际受污染的玉米粉样品中均证明了臭氧水溶液能有效降解ZEN，并预测了臭氧处理后ZEN的化学结构变化。考虑到形成的副产物的存在，臭氧处理前后暴露于ZEN的小鼠毒性效应的差异强调了进一步研究臭氧处理后实际食品样品的质量和毒性的重要性。
