Dong Jing, Liu Yongtao, Xu Ning, Yang Qiuhong, Ai Xiaohui
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China.
Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing, China.
Front Microbiol. 2018 Nov 21;9:2828. doi: 10.3389/fmicb.2018.02828. eCollection 2018.
() is an opportunistic bacterial pathogen widely distributed in the environments, particular aquatic environment. The pathogen can cause a range of infections in both human and animals including fishes. However, the application of antibiotics in treatment of infections leads to the emergence of resistant strains. Consequently, new approaches need to be developed in fighting this pathogen. Aerolysin, the chief virulence factor produced by pathogenic strains has been employed as target identifying new drugs. In our present study, we found that morin, a flavonoid without anti-bacterial activity isolated from traditional Chinese medicine, could directly inhibit the hemolytic activity of aerolysin. To determine the binding sites and the action of mechanism of morin against AerA, several assays were performed. Ser36, Pro347, and Arg356 were identified as the main binding sites affecting the conformation of AerA and resulted in block of the heptameric formation. Moreover, morin could protect Vero cells from cell injury mediated by aerolysin. study showed that morin could provide a protection to channel catfish against infection. These results demonstrated that morin could be developed as a promising candidate for the treatment of infections by decreasing the pathogenesis of .
（某菌）是一种机会性细菌病原体，广泛分布于环境中，尤其是水生环境。该病原体可导致人类和动物（包括鱼类）发生一系列感染。然而，使用抗生素治疗感染会导致耐药菌株的出现。因此，需要开发新的方法来对抗这种病原体。气溶素是致病性（某菌）菌株产生的主要毒力因子，已被用作鉴定新药的靶点。在我们目前的研究中，我们发现桑色素，一种从中药中分离出的无抗菌活性的黄酮类化合物，可直接抑制气溶素的溶血活性。为了确定桑色素对AerA的结合位点和作用机制，我们进行了几项试验。Ser36、Pro347和Arg356被确定为影响AerA构象并导致七聚体形成受阻的主要结合位点。此外，桑色素可以保护Vero细胞免受气溶素介导的细胞损伤。研究表明，桑色素可以为斑点叉尾鮰提供针对（某菌）感染的保护。这些结果表明，桑色素有望通过降低（某菌）的致病性而被开发为治疗（某菌）感染的候选药物。
