Dong Jing, Yan Tianhui, Yang Qiuhong, Zhou Shun, Song Yi, Liu Yongtao, Ma Liang, Xu Ning, Yang Yibin, Ai Xiaohui
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China.
College of Food Science and Engineering, Bohai University, Jinzhou, China.
Front Vet Sci. 2022 Jul 14;9:937463. doi: 10.3389/fvets.2022.937463. eCollection 2022.
The fast-growing demand for aquatic products has led to the rapid development of aquaculture. However, diseases caused by bacterial pathogens result in severe economic losses all over the world. Although the introduction of antibiotics to aquaculture decreased the mortality of infectious diseases, the emergence of antibiotic resistance caused treatment failure. Therefore, drugs with novel strategies are needed for combatting infections caused by resistant bacterial strains. In the present study, aerolysin was identified as a target for developing drugs from natural compounds against () infections. We found that polydatin without an inhibitory effect against growth could decrease the hemolysis mediated by aerolysin. In both western blot and qPCR assays, the addition of polydatin decreased the production of aerolysin by downregulating the aerolysin encoding gene. Moreover, cell viability and animal studies found that polydatin could reduce the pathogenesis of both and . Taken together, these findings provided a novel approach and candidate for treating resistant infections in aquaculture.
对水产品迅速增长的需求推动了水产养殖业的快速发展。然而，由细菌病原体引起的疾病在全球范围内造成了严重的经济损失。尽管在水产养殖中使用抗生素降低了传染病的死亡率，但抗生素耐药性的出现导致治疗失败。因此，需要具有新策略的药物来对抗由耐药菌株引起的感染。在本研究中，气溶素被确定为从天然化合物开发抗（）感染药物的靶点。我们发现，对（）生长没有抑制作用的虎杖苷可以减少气溶素介导的溶血作用。在蛋白质免疫印迹和定量聚合酶链反应分析中，添加虎杖苷通过下调气溶素编码基因来减少气溶素的产生。此外，细胞活力和动物研究发现，虎杖苷可以降低（）和（）的致病性。综上所述，这些发现为治疗水产养殖中的耐药（）感染提供了一种新方法和候选药物。
