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通过富含生育三烯酚的组分靶向肌微RNA以促进成肌细胞分化。

Targeting myomiRs by tocotrienol-rich fraction to promote myoblast differentiation.

作者信息

Razak Azraul Mumtazah, Khor Shy Cian, Jaafar Faizul, Karim Norwahidah Abdul, Makpol Suzana

机构信息

Department of Biochemistry, Faculty of Medicine, Level 17, Preclinical Building, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Jalan Yaakob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia.

出版信息

Genes Nutr. 2018 Nov 29;13:31. doi: 10.1186/s12263-018-0618-2. eCollection 2018.

DOI:10.1186/s12263-018-0618-2
PMID:30519366
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6267085/
Abstract

BACKGROUND

Several muscle-specific microRNAs (myomiRs) are differentially expressed during cellular senescence. However, the role of dietary compounds on myomiRs remains elusive. This study aimed to elucidate the modulatory role of tocotrienol-rich fraction (TRF) on myomiRs and myogenic genes during differentiation of human myoblasts. Young and senescent human skeletal muscle myoblasts (HSMM) were treated with 50 μg/mL TRF for 24 h before and after inducing differentiation.

RESULTS

The fusion index and myotube surface area were higher ( < 0.05) on days 3 and 5 than that on day 1 of differentiation. Ageing reduced the differentiation rate, as observed by a decrease in both fusion index and myotube surface area in senescent cells ( < 0.05). Treatment with TRF significantly increased differentiation at days 1, 3 and 5 of young and senescent myoblasts. In senescent myoblasts, TRF increased the expression of and and decreased and expression However, the expression of was upregulated during early differentiation and decreased at late differentiation when treated with TRF. In young myoblasts, TRF promoted differentiation by modulating the expression of , which resulted in the reduction of expression and upregulation of .

CONCLUSION

TRF can potentially promote myoblast differentiation by modulating the expression of myomiRs, which regulate the expression of myogenic genes.

摘要

背景

几种肌肉特异性微小RNA（肌微小RNA）在细胞衰老过程中存在差异表达。然而，膳食化合物对肌微小RNA的作用仍不明确。本研究旨在阐明富含生育三烯酚的组分（TRF）在人成肌细胞分化过程中对肌微小RNA和生肌基因的调节作用。在诱导分化前后，用50μg/mL TRF处理年轻和衰老的人骨骼肌成肌细胞（HSMM）24小时。

结果

分化第3天和第5天的融合指数和肌管表面积高于分化第1天（<0.05）。衰老降低了分化率，衰老细胞中的融合指数和肌管表面积均下降（<0.05）。TRF处理显著增加了年轻和衰老成肌细胞在第1、3和5天的分化。在衰老成肌细胞中，TRF增加了 和 的表达，并降低了 和 的表达。然而，在用TRF处理时， 的表达在早期分化过程中上调，在晚期分化时下降。在年轻成肌细胞中，TRF通过调节 的表达促进分化，这导致 表达降低和 表达上调。

结论

TRF可能通过调节肌微小RNA的表达来促进成肌细胞分化，而肌微小RNA可调节生肌基因的表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/44c3f855a3e7/12263_2018_618_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/6753b0bb237a/12263_2018_618_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/130190a7c617/12263_2018_618_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/3265b1f50a4b/12263_2018_618_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/25c964ffef2c/12263_2018_618_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/aba6e41b3607/12263_2018_618_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/bd871cdd5c10/12263_2018_618_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/137be2571f3e/12263_2018_618_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/44c3f855a3e7/12263_2018_618_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/6753b0bb237a/12263_2018_618_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/130190a7c617/12263_2018_618_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/3265b1f50a4b/12263_2018_618_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/25c964ffef2c/12263_2018_618_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/aba6e41b3607/12263_2018_618_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/bd871cdd5c10/12263_2018_618_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/137be2571f3e/12263_2018_618_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3388/6267085/44c3f855a3e7/12263_2018_618_Fig8_HTML.jpg

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Sci Rep. 2018 Jul 11;8(1):10471. doi: 10.1038/s41598-018-28708-z.
2
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Oxid Med Cell Longev. 2017;2017:3868305. doi: 10.1155/2017/3868305. Epub 2017 Jan 24.
3
γ-Tocotrienol prevents cell cycle arrest in aged human fibroblast cells through p16 pathway.
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Evid Based Complement Alternat Med. 2020 Apr 29;2020:1787342. doi: 10.1155/2020/1787342. eCollection 2020.
4
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Nutrients. 2020 Jan 22;12(2):297. doi: 10.3390/nu12020297.
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J Physiol Biochem. 2017 Feb;73(1):59-65. doi: 10.1007/s13105-016-0524-2. Epub 2016 Oct 14.
4
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5
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