Mohd Sahardi Nur Fatin Nabilah, Jaafar Faizul, Mad Nordin Mariam Firdhaus, Makpol Suzana
Department of Biochemistry, Faculty of Medicine, Level 17, Preclinical Building, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia.
Department of Chemical Process Engineering, Universiti Teknologi Malaysia (UTM) Kuala Lumpur, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, Malaysia.
Evid Based Complement Alternat Med. 2020 Apr 29;2020:1787342. doi: 10.1155/2020/1787342. eCollection 2020.
Ageing resulted in a progressive loss of muscle mass and strength. Increased oxidative stress in ageing affects the capacity of the myoblast to differentiate leading to impairment of muscle regeneration. Roscoe (ginger) has potential benefits in reversing muscle ageing due to its antioxidant property. This study aimed to determine the effect of ginger in the prevention of cellular senescence and promotion of muscle regeneration.
Myoblast cells were cultured into young and senescent state before treated with different concentrations of ginger standardised extracts containing different concentrations of 6-gingerol and 6-shogaol. Analysis on cellular morphology and myogenic purity was carried out besides determination of SA--galactosidase expression and cell cycle profile. Myoblast differentiation was quantitated by determining the fusion index, maturation index, and myotube size.
Treatment with ginger extracts resulted in improvement of cellular morphology of senescent myoblasts which resembled the morphology of young myoblasts. Our results also showed that ginger treatment caused a significant reduction in SA--galactosidase expression on senescent myoblasts indicating prevention of cellular senescence, while cell cycle analysis showed a significant increase in the percentage of cells in the G/G phase and reduction in the S-phase cells. Increased myoblast regenerative capacity was observed as shown by the increased number of nuclei per myotube, fusion index, and maturation index.
Ginger extracts exerted their potency in promoting muscle regeneration as indicated by prevention of cellular senescence and promotion of myoblast regenerative capacity.
衰老导致肌肉质量和力量逐渐丧失。衰老过程中氧化应激增加会影响成肌细胞的分化能力，进而导致肌肉再生受损。由于具有抗氧化特性，罗斯科姜（姜）在逆转肌肉衰老方面具有潜在益处。本研究旨在确定姜在预防细胞衰老和促进肌肉再生方面的作用。
将成肌细胞培养成年轻和衰老状态，然后用含有不同浓度6-姜酚和6-姜烯酚的不同浓度姜标准化提取物进行处理。除了测定β-半乳糖苷酶表达和细胞周期谱外，还对细胞形态和肌源性纯度进行了分析。通过测定融合指数、成熟指数和肌管大小来定量成肌细胞分化。
用姜提取物处理可改善衰老成肌细胞的细胞形态，使其类似于年轻成肌细胞的形态。我们的结果还表明，姜处理可使衰老成肌细胞上的β-半乳糖苷酶表达显著降低，表明预防了细胞衰老，而细胞周期分析显示G0/G1期细胞百分比显著增加，S期细胞减少。观察到成肌细胞再生能力增强，表现为每个肌管的核数量、融合指数和成熟指数增加。
如预防细胞衰老和促进成肌细胞再生能力所示，姜提取物在促进肌肉再生方面发挥了作用。
