Laboratory of Polymers, Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, EEL-USP, Estrada Municipal do Campinho, s/n, 12602-810, Lorena, SP, Brazil.
Laboratory of Polymers, Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, EEL-USP, Estrada Municipal do Campinho, s/n, 12602-810, Lorena, SP, Brazil.
Colloids Surf B Biointerfaces. 2019 Mar 1;175:73-83. doi: 10.1016/j.colsurfb.2018.11.042. Epub 2018 Nov 22.
In this paper, chitosan was used as protective agent for dual temperature-/pH-sensitive poly(N-vinylcaprolactam-co-itaconic acid-co-ethylene- glycol dimethacrylate)- based hydrogel nanoparticles (poly(NVCL-co-IA-co-EGDMA)) aiming avoid their undesirable colloidal destabilization at different conditions of body human tissues. Thus, poly(NVCL-co-IA-co-EGDMA) was embedded into chitosan and a new solid dispersion was prepared via spray-drying and ketoprofen was used as carrier. Two different sizes of hydrogel nanoparticles (120.6 nm and 185.9 nm) were evaluated and they exhibited a drug encapsulation efficiency of the 39.6% and 57.8%, respectively. The smaller nanoparticles showed to be faster for releasing of ketoprofen at pH 7.4 and 37 °C due to their larger surface area and higher swelling ability. Chitosan played a role of a secondary barrier for the ketoprofen diffusion, extending its release compared to hydrogel nanoparticles alone. Among two concentrations (40 wt% and 70 wt%) of hydrogel nanoparticles related to chitosan, the first one induced higher percentages of ketoprofen release: 74.2% against 64.6%. In addition, the interactions between chitosan matrix and poly(NVCL-co-IA-co-EGDMA) did not change the multi-responsive behavior of hydrogels, suggesting the chitosan was efficient for keeping integrity of nanoparticles hydrogels. Chitosan/poly(NVCL-co-IA-co-EGDMA) hybrid microparticles seems to be a promising new carrier for release of hydrophobic drugs, such as ketoprofen.
本文使用壳聚糖作为双重温度-/pH 敏感的聚（N-乙烯基己内酰胺-co-衣康酸-co-乙二醇二甲基丙烯酸酯）基水凝胶纳米粒子（poly（NVCL-co-IA-co-EGDMA））的保护剂，旨在避免其在人体组织不同条件下的胶体不稳定。因此，将 poly（NVCL-co-IA-co-EGDMA）嵌入壳聚糖中，并通过喷雾干燥制备新的固体分散体，其中酮洛芬被用作载体。评估了两种不同尺寸的水凝胶纳米粒子（120.6nm 和 185.9nm），它们分别显示出 39.6%和 57.8%的药物包封效率。较小的纳米粒子在 pH 7.4 和 37°C 下显示出更快的酮洛芬释放速度，这是由于它们具有更大的表面积和更高的溶胀能力。壳聚糖在酮洛芬扩散方面起到了二级屏障的作用，与单独的水凝胶纳米粒子相比，延长了其释放时间。在与壳聚糖相关的两种浓度（40wt%和 70wt%）的水凝胶纳米粒子中，第一种浓度诱导了更高的酮洛芬释放百分比：74.2%对 64.6%。此外，壳聚糖基质与 poly（NVCL-co-IA-co-EGDMA）之间的相互作用并没有改变水凝胶的多响应行为，这表明壳聚糖有效地保持了纳米水凝胶的完整性。壳聚糖/poly（NVCL-co-IA-co-EGDMA）杂化微球似乎是一种有前途的新型载体，可用于释放疏水性药物，如酮洛芬。
