Taskiran Mehmet, Tasdemir Abdulkadir, Ayyildiz Nusret, Ayyildiz Mustafa, Agar Erdal
a Department of Biology, Faculty of Science , Erciyes University , Kayseri , Turkey.
b Graduate School of Natural and Applied Sciences , Erciyes University , Kayseri , Turkey.
Int J Neurosci. 2019 Jul;129(7):687-697. doi: 10.1080/00207454.2018.1557166. Epub 2019 Jan 1.
This study was aimed at examining the epileptiform activity of the 5-HT serotonin receptor agonist and antagonist, and 5-hydroxytryptophan (5-HTP) in penicillin-induced epilepsy in albino Wistar rats.
For this purpose, 90 albino male Wistar rats were used in this study. Epileptiform activity was induced by an injection of penicillin, an agonist of GABAA receptor, (500 IU, i.c.) into the somatomotor cortex. Thirty minutes after the injection of penicillin, 2,5-dimethoxy-4-iodoamphetamine (DOI, an agonist of 5-HT receptor) (0.5, 1, 2 and 4 mg/kg, i.p.), methysergide, an antagonist of 5-HT receptor, (1, 10, 20, 50 and 100 µM, i.c.v.) and 5-HTP, precursor of 5-HT, (25, 50, 75 and 100 mg/kg, i.p.) were administered, respectively.
DOI, at the doses of 1 and 2 mg/kg, significantly decreased penicillin-induced epileptiform activity (p < 0.05). Methysergide, at the doses of 20, 50 and 100 µM, significantly increased the mean spike frequency of penicillin-induced epileptiform activity (p < 0.05). The doses of 50, 75 and 100 mg/kg of 5-HTP decreased the mean spike frequency of penicillin-induced epileptiform activity (p < 0.05). The mean of amplitude of penicillin-induced epileptiform activity did not significantly change in any of the groups (p > 0.05).
The electrophysiological data from the present study suggest that serotonin 5-HT receptors have an important role in controlling penicillin-induced epileptiform activity in the rat.
本研究旨在检测5-羟色胺（5-HT）血清素受体激动剂、拮抗剂及5-羟色氨酸（5-HTP）对白化Wistar大鼠青霉素诱发癫痫的癫痫样活动的影响。
本研究使用了90只白化雄性Wistar大鼠。通过向躯体运动皮层注射GABAA受体激动剂青霉素（500国际单位，脑内注射）诱发癫痫样活动。注射青霉素30分钟后，分别腹腔注射5-HT受体激动剂2,5-二甲氧基-4-碘苯丙胺（DOI，0.5、1、2和4毫克/千克）、5-HT受体拮抗剂麦角新碱（1、10、20、50和100微摩尔，脑室内注射）以及5-HT的前体5-HTP（25、50、75和100毫克/千克，腹腔注射）。
剂量为1和2毫克/千克的DOI显著降低了青霉素诱发的癫痫样活动（p<0.05）。剂量为20、50和100微摩尔的麦角新碱显著增加了青霉素诱发的癫痫样活动的平均棘波频率（p<0.05）。剂量为50、75和100毫克/千克的5-HTP降低了青霉素诱发的癫痫样活动的平均棘波频率（p<0.05）。在任何一组中，青霉素诱发的癫痫样活动的平均幅度均无显著变化（p>0.05）。
本研究的电生理数据表明，血清素5-HT受体在控制大鼠青霉素诱发的癫痫样活动中起重要作用。
