Ondokuz Mayıs University, Faculty of Yasar Dogu Sports Sciences, Samsun, Turkey.
Ondokuz Mayıs University, Faculty of Yasar Dogu Sports Sciences, Samsun, Turkey.
Epilepsy Behav. 2020 Nov;112:107403. doi: 10.1016/j.yebeh.2020.107403. Epub 2020 Sep 17.
Previous studies have shown that 5- hydroxytryptophan (5-HTP) and exercise play an important role in the synthesis of serotonin independently. The effects of the treadmill exercise and 5- hydroxytryptophan (5-HTP) on seizure mechanisms created by epileptiform activity with penicillin model were investigated in rats.
A total of 28 male albino Wistar rats were randomly divided into four groups: exercise (Ex), Control (Cnt), 5-hydroxytryptophan (5htp) and 5-hydroxytryptophan + exercise (5htpEx) groups. Treadmill exercise and gavage (25 mg/kg/day) were administered five days a week for ten weeks. Electrocorticogram data were recorded for 3 h at the end of the protocol using the Power-Lab data acquisition system. Spike frequency, amplitude, and latency time were analyzed offline. The significant differences among the groups were evaluated by one-way analysis of variance (ANOVA).
Spike frequency was observed at the highest level from the 20th minute in the Cnt group, and this continued until the end of the recording. The 5-HTP alone group did not affect epileptiform activity. At the 80th minute of penicillin injection, the epileptiform activity in the 5htpEx group decreased significantly compared with the Cnt, and this significance continued until the 110th minute. There was no statistical difference in the amplitude values of the groups. The 5htpEx group was significantly higher than both the Cnt and Ex group in the seizure latency times.
It was determined that exercise reduced the spike number and delayed seizure significantly by potentiating the effect of 5-HTP. Given that 5-HTP used in combination with exercise can perform useful actions such as reducing seizure sensitivity and consequently improving the quality of life for individuals with epilepsy, it may be a potential candidate for the treatment of epilepsy in nonpharmacological methods.
先前的研究表明，5-羟色氨酸（5-HTP）和运动各自在血清素的合成中起着重要作用。本研究旨在探讨电抽搐模型中，戊四氮诱导癫痫发作时，跑台运动和 5-羟色氨酸（5-HTP）对癫痫发作机制的影响。
将 28 只雄性白化 Wistar 大鼠随机分为 4 组：运动（Ex）组、对照组（Cnt）、5-羟色氨酸（5-HTP）组和 5-羟色氨酸+运动（5-HTPEx）组。每周运动 5 天，每天跑台运动，灌胃 5-HTP（25mg/kg/天）。在方案结束时，使用 Power-Lab 数据采集系统记录 3 小时的脑电数据。离线分析尖峰频率、幅度和潜伏期。采用单因素方差分析（ANOVA）比较组间差异。
Cnt 组在第 20 分钟时尖峰频率达到最高水平，并且持续到记录结束。5-HTP 单独处理组对癫痫样活动没有影响。在青霉素注射后第 80 分钟，5-HTPEx 组的癫痫样活动明显低于 Cnt 组，这种显著性一直持续到第 110 分钟。组间振幅值无统计学差异。5-HTPEx 组的发作潜伏期明显长于 Cnt 组和 Ex 组。
运动通过增强 5-HTP 的作用，显著降低了尖峰数量和发作次数。鉴于 5-HTP 与运动结合使用可以减少癫痫发作的敏感性，从而提高癫痫患者的生活质量，因此它可能是一种非药物治疗癫痫的潜在候选药物。
