Njoto Ibrahim, Soekanto Ayly, Ernawati Ernawati, Abdurrachman Abdurrachman, Kalim Handono, Handono Kusworini, Soeatmadji Djoko W, Fatchiyah Fatchiyah
Anatomy Department, Faculty of Medicine, Wijaya Kusuma Surabaya University.
Farmacology Department, Faculty of Medicine, Wijaya Kusuma Surabaya University.
Med Arch. 2018 Nov;72(5):348-351. doi: 10.5455/medarh.2018.72.348-351.
Chondrocyte is one cell in articular cartilage was products many proteins, molecules, and other factors. The external influence of cartilage, such as: hyperglycemia was entering joint capsule and impact to the chondrocytes and the cartilage. Hyperglycemia caused modification of heparan sulfate proteoglycan 2 (perlecan) proteins through glycation process.
The aim of this study was to analyze morphological changing of chondrocytes pericellular matrix by the influence of hyperglycemia.
Eighteen adult male rats were divided into six groups: control, rat treated with sugar intake was 0.5 mg/kg, 0.75 mg/ kg, 1mg/kg, 1.5 g/kg and 2 mg/kg of body weight. The animal model was dislocated and left knee was taken to observe changing of chondrocytes pericellular matrix strain fields by Scanning Electron Microscope (SEM) from perpendicular to femoral condylus cartilage.
Changing of chondrocytes intracellular matrix strain fields as changing of cell diameters and cell distances at group control and group I to group V, which cell diameters was lower level and cell distances was the highest level at over diet 2. This changing of intracellular matrix strain fields was corresponding to changing chondrocytes morphology in hyperglycemia condition, due to hypertrophic stage as adaptive responses. This research as based on next research for accomplish of hyperglycemia influence to morphology articular cartilage changing to prevent degeneration of cartilage towards osteoarthritis.
Present study concludes that hyperglycemia influence to chondrocyte intracellular matrix strain fields changing.
软骨细胞是关节软骨中的一种细胞，可产生多种蛋白质、分子和其他因子。软骨受到的外部影响，如高血糖进入关节囊并对软骨细胞和软骨产生影响。高血糖通过糖基化过程导致硫酸乙酰肝素蛋白聚糖2（基底膜聚糖）蛋白发生修饰。
本研究旨在分析高血糖影响下软骨细胞周围基质的形态变化。
将18只成年雄性大鼠分为六组：对照组、糖摄入量分别为0.5mg/kg、0.75mg/kg、1mg/kg、1.5g/kg和2mg/kg体重的大鼠组。使动物模型脱臼，取出左膝，通过扫描电子显微镜（SEM）从垂直于股骨髁软骨的方向观察软骨细胞周围基质应变场的变化。
对照组以及第一组至第五组软骨细胞内基质应变场的变化表现为细胞直径和细胞间距的改变，在饮食量超过2时，细胞直径处于较低水平，细胞间距处于最高水平。这种细胞内基质应变场的变化与高血糖条件下软骨细胞形态的变化相对应，这是由于肥大阶段作为适应性反应所致。本研究是基于后续研究，以完成高血糖对关节软骨形态变化的影响，从而预防软骨向骨关节炎的退变。
本研究得出结论，高血糖会影响软骨细胞内基质应变场的变化。
